| Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from rat liver. | |
| | |
MedLine Citation:
|
PMID: 624713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An in vitro study of bile acid-CoA:amino acid N-acyltransferase activity of rat liver was undertaken in order to determine whether separate amino acid-specific enzymes catalyzed the formation of glycine and taurine conjugates of bile acids as postulated by others. Polyacrylamide gel electrophoresis of 200-fold purified enzyme localized the glycine- and taurine-dependent activities to a single band. Both activities were optimal at pH 7.8 and showed similar loss of activity at pH 6.0, pH 9.0, in the presence of 5,5'-dithiobis(2-nitrobenzoic acid), and at temperatures exceeding 50 degrees. With the purified fraction, Km for glycine was 31 mM and Km for taurine was 0.8 mM. Km for several bile acid-CoA substrates was approximately 20 micron and independent of the amino acid acceptor. Only amino acids with terminal alpha- or beta-amino groups were active as acyl acceptors. Acyl donors were limited to bile acid-CoA derivatives. The data support the conclusion that the rat has a single bile acid-CoA:amino acid N-acyltransferase. The substrate kinetics are consistent with previous observations that taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine. |
| | |
Authors:
|
P G Killenberg; J T Jordan |
Related Documents
:
|
582963 - Effects of cicloxilic acid on secretory function of the liver. ii. effect on biliary se... 2326473 - Iodinated contrast medium as an aid to gallstone dissolution with methyl tert-butyl eth... 10544953 - Increased susceptibility to peroxidation of vldl from non-insulin-dependent diabetic pa... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 253 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1978 Feb |
Date Detail:
|
Created Date: 1978-04-17 Completed Date: 1978-04-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1005-10 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acyltransferases
/
isolation & purification,
metabolism* Amino Acids Animals Bile / enzymology* Cholic Acids Kinetics Liver / enzymology* Male Rats Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Cholic Acids; EC 2.3.-/Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chemical properties and amino acid composition of beta1-bungarotoxin from the venom of Bungarus mult...
Next Document: Lysosomal alpha-D-mannosidase of rat liver. Purification and comparison with the golgi and cytosolic...