Document Detail


Purification and characterization of Warnericin RB4, anti-Alicyclobacillus bacteriocin, produced by Staphylococcus warneri RB4.
MedLine Citation:
PMID:  15971094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we characterized a bacteriocin, warnericin RB4, produced by Staphylococcus warneri RB4. Warnericin RB4 activity was completely inactivated by trypsin and actinase E. The activity was stable at 100 degrees C for 15 min, and had a pH range of 2 to 6. S. warneri RB4 showed antibacterial activity against only Alicyclobacillus acidoterrestris, A. acidocaldarius, and Micrococcus luteus, among 34 bacterial species tested. The amino acid sequence of the purified bacteriocin contained 27 amino acid residues (K-K-K-S-G-V-I-P-X-V-X-H-D-X-H-M-N-X-F-Q-F-V-F-X-X-X-S). The molecular mass of the bacteriocin was estimated to be 2,958.2 Da by ESI-MS. These results show that the Warnericin RB4 exhibiting specific antibacterial activity against thermo-acidophiles, Alicyclobacillus spp., is a Nukacin ISK-1 or closely related bacteriocin, classified with class IA (Lacticin 481 types). This is the first report that Warnericin RB-4 is effective to inhibit the growth of causative microorganisms of spoilage in various acidic drinks. Warnericin RB4 might prove useful in fruit juices and fruit juice-containing drinks.
Authors:
Misuzu Minamikawa; Yuji Kawai; Norio Inoue; Koji Yamazaki
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Publication Detail:
Type:  Journal Article     Date:  2005-06-16
Journal Detail:
Title:  Current microbiology     Volume:  51     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-08-31     Completed Date:  2005-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22-6     Citation Subset:  IM    
Affiliation:
Laboratory of Marine Food Science, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / biosynthesis,  isolation & purification,  pharmacology
Bacteriocins / biosynthesis*,  chemistry,  isolation & purification,  pharmacology
Food Preservation
Hydrogen-Ion Concentration
Staphylococcus / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins; 0/warnericin RB4, Staphylococcus warneri

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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