Document Detail

Purification and characterization of L-phenylalanine aminopeptidase from chick-pea cotyledons (Cicer arietinum L.).
MedLine Citation:
PMID:  19200046     Owner:  NLM     Status:  MEDLINE    
Chick-pea (Cicer arietinum L.) cotyledons are unique source of aminopeptidase - 8-9 U/g cotyledons was observed using L-leucine-p-nitroanilide as substrate. The aminopeptidase was purified (65 kDa, pI 4.8 ) reaching a specific activity of 220 U/mg at pH 7.0-7.2 and 35-40 degrees C. The determined constant of specificity k(cat)/K(m) during hydrolysis of N-unsubstituted amino acid-p-nitroanilides showed a decrease order: Phe>Leu>Pro>Ile>Val>Ala. The enzyme was strongly inhibited by p-chloromercuribenzoic acid as well as in a competitive rate by the antihypertensive peptides Ile-Pro-Pro and Val-Pro-Pro.
Margarita Marinova; Alexander Dolashki; Florian Altenberend; Stefan Stevanovic; Wolfgang Voelter; Bozhidar Tchorbanov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein and peptide letters     Volume:  16     ISSN:  0929-8665     ISO Abbreviation:  Protein Pept. Lett.     Publication Date:  2009  
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-12     Citation Subset:  IM    
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 9, 1113 Sofia, Bulgaria.
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MeSH Terms
Aminopeptidases / antagonists & inhibitors,  chemistry,  isolation & purification*,  metabolism*
Cicer / enzymology*
Cotyledon / enzymology*
Hydrogen-Ion Concentration
Oligopeptides / metabolism
Sequence Alignment
Substrate Specificity
Reg. No./Substance:
0/Oligopeptides; 0/isoleucyl-prolyl-proline; 0/valyl-prolyl-proline; EC 3.4.11.-/Aminopeptidases; EC 3.4.11.-/phenylalanine aminopeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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