Document Detail


Purification and characterization of KpsT, the ATP-binding component of the ABC-capsule exporter of Escherichia coli K1.
MedLine Citation:
PMID:  12855177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The K1 capsule, an alpha(2,8)-linked polymer of sialic acid, is an important virulence determinant of invasive Escherichia coli. The 17-kb kps gene cluster of E. coli K1 encodes the information necessary for capsule expression at the cell surface. Two proteins, KpsM and KpsT, play a role in the transport of capsular polysaccharide across the cytoplasmic membrane, utilizing the energy from ATP hydrolysis. They belong to the ATP-binding cassette superfamily of transport proteins. In this study, we purified KpsT in its native form and show that the purified protein is able to bind ATP, undergo an ATP-dependent conformational change and hydrolyze ATP. Protease accessibility studies demonstrate the in vivo interaction between KpsM and KpsT.
Authors:
Christiane J Nsahlai; Richard P Silver
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  224     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-11     Completed Date:  2003-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  113-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave., Box 672, Rochester, NY 14642, USA.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / chemistry,  genetics,  isolation & purification,  metabolism*
Adenosine Triphosphatases / metabolism
Adenosine Triphosphate / analogs & derivatives*,  metabolism,  pharmacokinetics
Azides / pharmacokinetics
Bacterial Capsules / metabolism*
Escherichia coli / genetics,  metabolism*
Escherichia coli Proteins / chemistry,  genetics,  isolation & purification,  metabolism*
Membrane Transport Proteins / metabolism
Phosphorus Radioisotopes / diagnostic use
Photochemistry
Plasmids
Protein Structure, Tertiary
Grant Support
ID/Acronym/Agency:
AI39615/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Azides; 0/Bacterial Capsules; 0/Escherichia coli Proteins; 0/Membrane Transport Proteins; 0/Phosphorus Radioisotopes; 133135-12-3/kpsM protein, E coli; 133135-13-4/kpsT protein, E coli; 53696-59-6/8-azidoadenosine 5'-triphosphate; 56-65-5/Adenosine Triphosphate; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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