Document Detail


Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom.
MedLine Citation:
PMID:  17056081     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crude venom from Thai giant scorpion, Heterometrus laoticus, most commonly found in the northeastern area of Thailand, was evaluated for PD50 (paralytic dose 50) activities from abdominal injection to cricket (Gryllus sp.) and activities against various kinds of microorganisms. It exhibited good results in disc diffusion assay for Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. After purification, toxin showed acceptable results for PD50 determination in entrapped cricket as well as inhibitory activity against B. subtilis, K. pneumoniae, and P. aeruginosa with activities over 300 times higher than that of the crude venom. The purified fraction was showed to contain a single band in SDS-PAGE. MALDI-TOF-MS/MS analysis showed one peak of major protein with 8293Da. Partial amino acid sequence show high homology to Scorpine-a polypeptide toxin family with potassium channel blocking and defensin activity. The novel toxin was named "Heteroscorpine-1" (HS-1) as the first Scorpine from genus Heterometrus. After full length determination by PCR, HS-1 gene was found to be composed of two exons, separated by an intron. Deduction revealed 95 amino acid residues with 19 residues as the leading sequence. It showed about 80% similarity to Panscorpine and Opiscorpine.
Authors:
Nunthawun Uawonggul; Sompong Thammasirirak; Arunrat Chaveerach; Tarinee Arkaravichien; Wandee Bunyatratchata; Wipaporn Ruangjirachuporn; Pornpimol Jearranaiprepame; Takeshi Nakamura; Michiyuki Matsuda; Michimoto Kobayashi; Seisuke Hattori; Sakda Daduang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-14
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  49     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-18     Completed Date:  2007-03-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  19-29     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Anti-Bacterial Agents / pharmacology
Antifungal Agents / pharmacology
Cloning, Molecular
DNA / analysis
DNA Primers
Gram-Negative Bacteria / drug effects,  ultrastructure
Gram-Positive Bacteria / drug effects,  ultrastructure
Injections
Insects / drug effects
Lethal Dose 50
Microbial Sensitivity Tests
Mitosporic Fungi / drug effects
Molecular Sequence Data
Polymerase Chain Reaction
Scorpion Venoms / chemistry,  isolation & purification,  pharmacology,  toxicity*
Scorpions*
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antifungal Agents; 0/DNA Primers; 0/Scorpion Venoms; 0/heteroscorpine-1, Heterometrus laoticus; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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