Document Detail

Purification and characterization of Clostridium botulinum type B oxin.
MedLine Citation:
PMID:  5327476     Owner:  NLM     Status:  MEDLINE    
Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, David V. Kason, and Jack H. Tremaine. Purification and characterization of Clostridium botulinum type B toxin. J. Bacteriol. 91:484-487. 1966.-A toxic component of low molecular weight has been isolated from a type B strain of Clostridium botulinum by methods involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. The material thus isolated was shown to be monophoretic and monodisperse in the ultracentrifuge. End-group analysis indicated the presence of a single N-terminal amino acid residue, which was identified as arginine. On the basis of biophysical studies and amino acid analyses, a molecular weight between 9,000 and 10,000 was calculated.
J Gerwing; C E Dolman; D V Kason; J H Tremaine
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  91     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1966 Feb 
Date Detail:
Created Date:  1966-08-13     Completed Date:  1966-08-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  484-7     Citation Subset:  IM    
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MeSH Terms
Amino Acids
Bacterial Proteins
Clostridium botulinum*
Molecular Weight
Toxins, Biological*
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Toxins, Biological; 74-79-3/Arginine

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