Document Detail


Purification and characterisation of four polypeptides with neurotoxic activity from Condylactis aurantiaca.
MedLine Citation:
PMID:  8368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four toxic polypeptides, Toxins I, II, III and IV were isolated in pure form from the sea anemone Condylactis aurantiaca (Actinaria). Toxin isolation was achieved by alcoholic extraction of the homogenised sea anemones, batchwise adsorption onto cation exchangers, gel filtration on Sephadex G-50 and G-25 and ion-exchange chromatography on SP-Sephadex and QAE-Sephadex. Toxins from Condylactis aurantiaca all contain between 49 and 51 amino acids. Their amino acid compositions were compared to those of the Anemonia sulcata toxins. The toxins were tested on the shore crab Carcinus maenas by intramusclar injection. Crabs react highly sensitively to sea anemone toxins with muscle cramps and paralysis. For Condylactis toxins LD100 ranges from 2 to 6.6 mug/kg Carcinus maenas.
Authors:
R Béress; L Béress; G Wunderer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hoppe-Seyler's Zeitschrift für physiologische Chemie     Volume:  357     ISSN:  0018-4888     ISO Abbreviation:  Hoppe-Seyler's Z. Physiol. Chem.     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-11-01     Completed Date:  1976-11-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985060R     Medline TA:  Hoppe Seylers Z Physiol Chem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  409-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Brachyura
Cnidaria / analysis*
Marine Toxins / analysis*,  isolation & purification
Peptides / analysis
Sea Anemones / analysis*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Marine Toxins; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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