Document Detail


Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics.
MedLine Citation:
PMID:  23683700     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1h incubation at 100°C whilst simultaneously showing kinetic adaptations to cold temperatures. LipG7 was also found to have industrial potential as an enantioselective biocatalyst as it is able to effectively catalyse the enantioselective transesterification of a secondary alcohol. The LipG7 coding sequence has been identified and cloned using 454 pyrosequencing of the transcriptome and inverse PCR. The LipG7 protein has been heterologously expressed in Saccharomyces cerevisiae BJ5465 and shown to exhibit the same characteristics as the native protein.
Authors:
Tomasz Florczak; Maurycy Daroch; Mark Charles Wilkinson; Aneta Białkowska; Andrew Derek Bates; Marianna Turkiewicz; Lesley Ann Iwanejko
Publication Detail:
Type:  Journal Article     Date:  2013-04-04
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  53     ISSN:  1879-0909     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
Affiliation:
Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Technical University of Lodz, ul. B. Stefanowskiego 4/10, 90-924 Lodz, Poland; Institute of Integrative Biology, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, United Kingdom.
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