| Purification of a chalone-like inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland. | |
| | |
MedLine Citation:
|
PMID: 6221930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A factor which inhibits the resumption of proliferation of stationary Ehrlich ascites mammary carcinoma (EAC) cells in vitro was isolated from the normal, lactating bovine mammary gland. After fractionation of the 50,000 g supernatant of the tissue homogenate by ammonium sulfate precipitation and gel filtration, it was further purified 2600-fold by ultrafiltration and lectin affinity chromatography. Polyacrylamide electrophoresis revealed one band containing all the activity. SDS gel electrophoresis showed a main band corresponding to a protein with a molecular weight of about 15,000 dalton. At this state of purity the factor concentration for half-maximal inhibition of proliferation was 12 ng/ml or 8 X 10(-10) M. The effects of factor concentrations of 4 X 10(-9) or 4 X 10(-8) M are abolished by 5 X 10(-9) M insulin almost completely or to 70%. |
| | |
Authors:
|
W Lehmann; R Samtleben; H Graetz; P Langen |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of cancer & clinical oncology Volume: 19 ISSN: 0277-5379 ISO Abbreviation: Eur J Cancer Clin Oncol Publication Date: 1983 Jan |
Date Detail:
|
Created Date: 1983-07-08 Completed Date: 1983-07-08 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8112045 Medline TA: Eur J Cancer Clin Oncol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 101-7 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carcinoma, Ehrlich Tumor / pathology* Cattle Cell Division / drug effects Cell Line Chromatography, Affinity Depression, Chemical Dose-Response Relationship, Drug Electrophoresis, Disc Electrophoresis, Polyacrylamide Gel Growth Inhibitors / isolation & purification*, pharmacology Insulin / pharmacology Mammary Glands, Animal / analysis* Mammary Neoplasms, Experimental / pathology* Ultrafiltration |
| Chemical | |
Reg. No./Substance:
|
0/Growth Inhibitors; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Experimental evaluation of three biomaterials for replacement of the pulmonary artery branches.
Next Document: Strong histocompatibility and cell-mediated cytotoxic effects of a single Mls difference demonstrate...