Document Detail


Purification of a chalone-like inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland.
MedLine Citation:
PMID:  6221930     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A factor which inhibits the resumption of proliferation of stationary Ehrlich ascites mammary carcinoma (EAC) cells in vitro was isolated from the normal, lactating bovine mammary gland. After fractionation of the 50,000 g supernatant of the tissue homogenate by ammonium sulfate precipitation and gel filtration, it was further purified 2600-fold by ultrafiltration and lectin affinity chromatography. Polyacrylamide electrophoresis revealed one band containing all the activity. SDS gel electrophoresis showed a main band corresponding to a protein with a molecular weight of about 15,000 dalton. At this state of purity the factor concentration for half-maximal inhibition of proliferation was 12 ng/ml or 8 X 10(-10) M. The effects of factor concentrations of 4 X 10(-9) or 4 X 10(-8) M are abolished by 5 X 10(-9) M insulin almost completely or to 70%.
Authors:
W Lehmann; R Samtleben; H Graetz; P Langen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  19     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Ehrlich Tumor / pathology*
Cattle
Cell Division / drug effects
Cell Line
Chromatography, Affinity
Depression, Chemical
Dose-Response Relationship, Drug
Electrophoresis, Disc
Electrophoresis, Polyacrylamide Gel
Growth Inhibitors / isolation & purification*,  pharmacology
Insulin / pharmacology
Mammary Glands, Animal / analysis*
Mammary Neoplasms, Experimental / pathology*
Ultrafiltration
Chemical
Reg. No./Substance:
0/Growth Inhibitors; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Experimental evaluation of three biomaterials for replacement of the pulmonary artery branches.
Next Document:  Strong histocompatibility and cell-mediated cytotoxic effects of a single Mls difference demonstrate...