| Purification and biological activity of the thrombin-like substance isolated from Bothrops insularis venom. | |
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MedLine Citation:
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PMID: 17161857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The venom of Bothrops insularis snake, known in Brazil as jararaca ilhoa, contains a variety of proteolytic enzymes such as a thrombin-like substance that is responsible for various pharmacological effects. B. insularis venom chromatography profile showed an elution of seven main fractions. The thrombin-like activity was detected in fractions I and III, the latter being subjected to two other chromatographic procedures, so to say DEAE and Hi Trap Benzamidine. The purity degree of this fraction was confirmed by analytical reverse phase HPLC, which displayed only one main fraction confirmed by SDS-PAGE constituting fraction III. About 5 microg of fraction III protein potentiated the secretion of insulin induced by 2.8 mM of glucose in rats isolated pancreatic beta-cells treated; the increase being around 3-fold higher than its respective control. B. insularis lectin (BiLec; 10 microg/mL) was also studied as to its effect on the renal function of isolated perfused rat kidneys with the use of six Wistar rats. BiLec increased perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF) and glomerular filtration rate (GFR). Sodium (%TNa+) and chloride tubular reabsorption (%TCl-) decreased at 120 min, without alteration in potassium transport. In conclusion, the thrombin-like substance isolated from B. insularis venom induced an increase in insulin secretion, in vitro, and transiently altered vascular, glomerular and tubular parameters in the isolated rat kidney. |
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Authors:
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Marcus Davis Machado Braga; Alice Maria Costa Martins; Dalgimar Bezerra de Menezes; Paulo Sergio Ferreira Barbosa; Janaina Serra Azul Monteiro Evangelista; Marcos Hikari Toyama; Daniela Oliveira Toyama; Manassés Claudino Fonteles; Helena Serra Azul Monteiro |
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Publication Detail:
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Type: Journal Article Date: 2006-10-25 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 49 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-05 Completed Date: 2007-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
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Languages: eng Pagination: 329-38 Citation Subset: IM |
Affiliation:
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Department of Pathology, Federal University of Ceara, Fortaleza, Ceara, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Coagulation / drug effects* Bothrops* Cells, Cultured Chlorides / metabolism Chromatography, High Pressure Liquid Crotalid Venoms / chemistry, isolation & purification*, pharmacology Electrophoresis, Polyacrylamide Gel Glomerular Filtration Rate / drug effects Hemostatics / chemistry, isolation & purification*, pharmacology Insulin / metabolism Insulin-Secreting Cells / drug effects, metabolism, pathology Kidney / blood supply, drug effects, pathology Kidney Tubules / drug effects, metabolism Lectins / pharmacology Male Perfusion Rats Rats, Wistar Urination / drug effects Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Crotalid Venoms; 0/Hemostatics; 0/Lectins; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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