| Purification and biological activity of acidic polysaccharide from leaves of Thymus vulgaris L. | |
MedLine Citation:
|
PMID: 11510490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polysaccharides are involved in biological responses and can activate complement system, which plays an important role in primary host defense mechanisms. We investigated anticomplementary activities from spice plants and selected thyme (Thymus vulgaris L.) as a potent complementary activator. Acidic polysaccharide (TV-3-IIIA-IIa) purified from the hot-water extract of thyme leaves by DEAE-Toyopearl 650C, Butyl-Toyopearl 650M and Sepharose CL-6B column chromatography and preparative HPLC. The purified polysaccharide, TV-3-IIIA-IIa showed potent anticomplementary activity via classical and alternative pathway with the increase proportional to dosage. TV-3-IIIA-IIa seemed to be a homogenous polymer from the results of HPLC and its molecular mass was estimated as 180 kDa. TV-3-IIIA-IIa mainly consisted of galacturonic acid (44.8 mol%), glucuronic acid (16.7 mol%), arabinose (11.1 mol%), rhamnose (9.2 mol%), galactose (8.9 mol%) and small amounts of glucose, xylose, mannose and fucose. By methylation analysis and reactivity to beta-glucosyl Yariv reagent, TV-3-IIIA-IIa was assumed to contain small amounts of type II arabinogalactan and large amounts of pectin-like polysaccharides in the structure. Based upon these results, TV-3-IIIA-IIa was suggested to be a complement activator. |
Authors:
|
H Chun; D H Shin; B S Hong; H Y Cho; H C Yang |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological & pharmaceutical bulletin Volume: 24 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-08-20 Completed Date: 2002-01-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
|
Languages: eng Pagination: 941-6 Citation Subset: IM |
Affiliation:
|
Graduate School of Biotechnology, Korea University, Seoul. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, DEAE-Cellulose Chromatography, High Pressure Liquid Complement Inactivator Proteins / pharmacology* Erythrocytes / drug effects Methylation Molecular Weight Plant Extracts / pharmacology Plant Leaves / chemistry Polysaccharides / isolation & purification*, pharmacology* Sheep Thymus Plant / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Complement Inactivator Proteins; 0/Plant Extracts; 0/Polysaccharides; 0/TV-3-IIIA-IIa |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular and pharmacokinetic properties of 222 commercially available oral drugs in humans.
Next Document: Identification of 16alpha,19-dihydroxyandrostenedione in the serum of pregnant women by gas chromato...