Document Detail


Purification and biological activity of acidic polysaccharide from leaves of Thymus vulgaris L.
MedLine Citation:
PMID:  11510490     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polysaccharides are involved in biological responses and can activate complement system, which plays an important role in primary host defense mechanisms. We investigated anticomplementary activities from spice plants and selected thyme (Thymus vulgaris L.) as a potent complementary activator. Acidic polysaccharide (TV-3-IIIA-IIa) purified from the hot-water extract of thyme leaves by DEAE-Toyopearl 650C, Butyl-Toyopearl 650M and Sepharose CL-6B column chromatography and preparative HPLC. The purified polysaccharide, TV-3-IIIA-IIa showed potent anticomplementary activity via classical and alternative pathway with the increase proportional to dosage. TV-3-IIIA-IIa seemed to be a homogenous polymer from the results of HPLC and its molecular mass was estimated as 180 kDa. TV-3-IIIA-IIa mainly consisted of galacturonic acid (44.8 mol%), glucuronic acid (16.7 mol%), arabinose (11.1 mol%), rhamnose (9.2 mol%), galactose (8.9 mol%) and small amounts of glucose, xylose, mannose and fucose. By methylation analysis and reactivity to beta-glucosyl Yariv reagent, TV-3-IIIA-IIa was assumed to contain small amounts of type II arabinogalactan and large amounts of pectin-like polysaccharides in the structure. Based upon these results, TV-3-IIIA-IIa was suggested to be a complement activator.
Authors:
H Chun; D H Shin; B S Hong; H Y Cho; H C Yang
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  24     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-20     Completed Date:  2002-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  941-6     Citation Subset:  IM    
Affiliation:
Graduate School of Biotechnology, Korea University, Seoul.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, DEAE-Cellulose
Chromatography, High Pressure Liquid
Complement Inactivator Proteins / pharmacology*
Erythrocytes / drug effects
Methylation
Molecular Weight
Plant Extracts / pharmacology
Plant Leaves / chemistry
Polysaccharides / isolation & purification*,  pharmacology*
Sheep
Thymus Plant / chemistry*
Chemical
Reg. No./Substance:
0/Complement Inactivator Proteins; 0/Plant Extracts; 0/Polysaccharides; 0/TV-3-IIIA-IIa

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