Document Detail

Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1.
MedLine Citation:
PMID:  18197839     Owner:  NLM     Status:  MEDLINE    
An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.
Xiao-Hong Cao; Zhen-Yu Liao; Chun-Ling Wang; Ping Cai; Wen-Yan Yang; Mei-Fang Lu; Guo-Wei Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  52     ISSN:  1470-8744     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-02     Completed Date:  2009-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
School of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
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MeSH Terms
Antineoplastic Agents / chemistry,  isolation & purification*,  metabolism,  pharmacology*
Apoptosis / drug effects
Bacillus / metabolism*
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Enzyme Activation / drug effects
L-Lactate Dehydrogenase / metabolism
Lipopeptides / biosynthesis,  chemistry,  isolation & purification*,  pharmacology*
Oils / chemistry
Spectrometry, Mass, Electrospray Ionization
Surface-Active Agents / chemistry,  isolation & purification*,  metabolism,  pharmacology*
Tandem Mass Spectrometry
Reg. No./Substance:
0/Antineoplastic Agents; 0/Lipopeptides; 0/Oils; 0/Surface-Active Agents; EC Dehydrogenase; EC 3.4.22.-/Caspase 3

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