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Purification and antitumor activity of two acidic polysaccharides from the roots of Polygala tenuifolia.
MedLine Citation:
PMID:  22944432     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Two acidic polysaccharide fractions (PTPa and PTPb) extracted from the roots of Polygala tenuifolia, were obtained by DEAE-Sephacel anion-exchange, and Sephadex G-100 gel-permeation chromatography. High-performance liquid chromatography (HPLC) identified that PTPa and PTPb was composed of Ara, Glc, Gal, Man and GlcUA in the proportion of 2.4:1.2:0.6:0.4:1.1 and 2.1:1.7:0.5:0.6:1.7, respectively. Their molecular weight was evaluated to be 5.9×10(4) (PTPa) and 2.5×10(4)Da (PTPb) as determined by high performance size exclusion chromatography (HPSEC). Pharmacological studies revealed PTPa and PTPb significantly inhibited the growth of A549 cells in vitro and exhibited significantly higher antitumor activity against solid tumor A549 in vivo than did a blank control. Moreover, treatment with two acidic polysaccharides caused an enhancement of superoxide dismutase (SOD) and catalase (CAT) activities in tumor-bearing mice and a reduction in thiobarbituric acid reactive substances (TBARS) level. Taken together, these results indicated that two acidic polysaccharides from the roots of P. tenuifolia may be useful as potent antitumor agents for the prevention of lung tumorigenesis.
Authors:
Tao Xin; Fubin Zhang; Qiuying Jiang; Chunhong Chen; Dayong Huang; Yanju Lv; Weixi Shen; Yinghua Jin
Publication Detail:
Type:  Journal Article     Date:  2012-07-27
Journal Detail:
Title:  Carbohydrate polymers     Volume:  90     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  1671-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Medical Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.
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