Document Detail


Purification of an antifungal compound, cyclo(l-Pro-d-Leu) for cereals produced by Bacillus cereus subsp. thuringiensis associated with entomopathogenic nematode.
MedLine Citation:
PMID:  23305769     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mold spoilage is the main cause of substantial economic loss in cereals and might also cause public health problems due to the production of mycotoxins. The aim of this study was to separate and purify and to identify antifungal compounds of bacterium associated with novel entomopathogenic nematode and check the antifungal property of identified compound in particular food model systems. The antifungal compound was purified using silica gel column chromatography, TLC and HPLC and its structure was elucidated using NMR ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, (1)H-(13)C HMBC), HRMS and Marfey's method. Based on the spectral data, the active compounds were identified as diketopiperazine [cyclo(l-Pro-d-Leu)]. The antifungal activity of cyclo(l-Pro-d-Leu) was studied by MIC and paper disk assay against Aspergillus flavus MTCC 277 and Aspergillus niger MTCC 282 and best MIC value of 8μg/ml was recorded against A. flavus. Cyclo(l-Pro-d-Leu) strongly inhibit mycelia growth of fungus and thereby affecting aflatoxin production. To investigate the potential application of the cyclo(l-Pro-d-Leu) and to eliminate fungal spoilage in food and feed, soybean and peanut were used as models. White mycelia and dark/pale green spores of A. flavus were observed in the control soybeans after 2-day incubation. However the fungal growth was not observed in soybeans treated with cyclo(l-Pro-d-Leu). Almost the same result was observed for peanuts treated with cyclo(l-Pro-d-Leu) for A. niger. The cyclo(l-Pro-d-Leu) was nontoxic to two normal human cell lines (FS normal fibroblast and L231 lung epithelial) up to 200μg/ml. Thus the diketopiperazine derivative identified in the study may be a promising alternative to chemical preservatives as a potential biopreservative which prevent fungal growth and mycotoxin formation in food and feed.
Authors:
S Nishanth Kumar; C Mohandas; Bala Nambisan
Related Documents :
22480379 - Loss of trophic complexity in saline prairie lakes as indicated by stable-isotope based...
21843789 - The effects of different ecophysiological factors on ochratoxin a production.
22498079 - Food motivation circuitry hypoactivation related to hedonic and nonhedonic aspects of h...
22751269 - Orthorexia nervosa behavior in a sample of brazilian dietitians assessed by the portugu...
23564589 - Study of the toxic effects of flame retardant pbde-47 on the clam chamelea gallina (lin...
24721259 - Activity of thymus vulgaris essential oil against anisakis larvae.
5574329 - Occurrence of vibrio parahaemolyticus in estuarine waters and oysters of new hampshire.
11160579 - Choose a variety of grains daily, especially whole grains: a challenge for consumers.
21355749 - Dietary intake, zincemia and cupremia of cameroonian schoolchildren of ngali ii.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-7
Journal Detail:
Title:  Microbiological research     Volume:  -     ISSN:  1618-0623     ISO Abbreviation:  Microbiol. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9437794     Medline TA:  Microbiol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier GmbH. All rights reserved.
Affiliation:
Division of Crop Protection/Division of Crop Utilization, Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695017, India. Electronic address: nishanthctcri@gmail.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Construction of a gentamicin C1a-overproducing strain of Micromonospora purpurea by inactivation of ...
Next Document:  In vivo gene expression and immunoreactivity of Leptospira collagenase.