| Purification of adrenal medullary chromaffin cells by density gradient centrifugation. | |
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MedLine Citation:
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PMID: 7329096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method has been developed for the isolation of chromaffin cells from guinea pig adrenal glands. Crude suspensions of adrenal cells are prepared by the digestion of adrenal with collagenase. Chromaffin cells are then purified from these crude suspensions by isopycnic centrifugation through a 5-25% (w/v) gradient of metrizamide. More than 90% of the cells in the preparation are viable chromaffin cells, as judged by trypan blue exclusion and fluorescence histochemistry. A more convenient method for monitoring the purity of chromaffin cell preparations, using neutral red staining, is also described. Chromaffin cells are the primary neutral red staining cell in the adrenal gland, and more than 90% of the purified chromaffin cells stain with this dye. Purified chromaffin cells contain 400 +/- 50 nmol of epinephrine/mg protein (133 +/- 16 nmol/10(6) cells), and secrete epinephrine in response to acetylcholine. At a concentration of 100 microM, acetylcholine causes a 10- to 20-fold increase in the secretion of epinephrine from the cells. The method described in this paper is a useful procedure for the preparation of pure, functional chromaffin cells. |
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Authors:
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L W Role; R L Perlman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 2 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 1980 Jun |
Date Detail:
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Created Date: 1982-04-22 Completed Date: 1982-04-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 253-65 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Medulla
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cytology* Animals Cell Separation / methods* Centrifugation, Density Gradient / methods* Chromaffin System / cytology* Enterochromaffin Cells / cytology* Epinephrine / analysis Guinea Pigs |
| Grant Support | |
ID/Acronym/Agency:
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HL 18148/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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51-43-4/Epinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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