Document Detail


Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system.
MedLine Citation:
PMID:  9258432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of induction. A purification scheme is described that produced 50 mg of RRF from an initial culture of 2 L. The recycling time for ribosomes synthesising the tripeptide fMet-Phe-Leu in vitro in the absence of RF3 was reduced from 40 to 15 s by the addition of purified 1.5 microM RRF.
Authors:
J MacDougall; P Holst-Hansen; K K Mortensen; D V Freistroffer; M Y Pavlov; M Ehrenberg; R H Buckingham
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  79     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  243-6     Citation Subset:  IM    
Affiliation:
CNRS-UPR 9073, Institut de Biologie Physico-chimique, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis,  genetics,  isolation & purification*,  metabolism
Cloning, Molecular
Escherichia coli / chemistry*,  genetics
Proteins*
Recombinant Proteins / genetics,  isolation & purification
Ribosomal Proteins
Water-Electrolyte Balance
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Proteins; 0/Recombinant Proteins; 0/Ribosomal Proteins; 0/ribosome releasing factor

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