| Purification of an acidic recombinant protein from transgenic tobacco. | |
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MedLine Citation:
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PMID: 17787005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tobacco has proven to be a promising alternative for the production of recombinant therapeutic proteins and offers numerous advantages over other plants as a host system. However, the recovery and purification steps needed to obtain a protein at high recovery and purity have not been well investigated. In this study, a process was developed to purify a model acidic protein, recombinant beta-glucuronidase (rGUS) from transgenic tobacco leaf tissue, in three main steps after extraction: polyelectrolyte precipitation, hydrophobic interaction chromatography (HIC), and hydroxyapatite chromatography (HAC). Using this three-step process, up to 40% of the initial rGUS activity could be recovered to near homogeneity as judged by SDS-PAGE. This work demonstrates that acidic recombinant proteins expressed in tobacco may be purified to high yield with high purity in a minimal amount of steps that are suitable for scale-up. Furthermore, the general steps used in this process may suggest that a wide variety of acidic recombinant proteins may be purified in a similar manner from transgenic tobacco or other leafy crops. |
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Authors:
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Chris Holler; Chenming Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 99 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-01-31 Completed Date: 2008-02-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 902-9 Citation Subset: IM |
Copyright Information:
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Copyright 2007 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, Virginia 24061, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography
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methods Glucuronidase / isolation & purification*, physiology* Plant Leaves / metabolism* Plants, Genetically Modified / metabolism* Recombinant Proteins / isolation & purification*, metabolism* Tobacco / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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