Document Detail


Purification of acid phosphatase I from germinating seeds of Vigna sinensis.
MedLine Citation:
PMID:  8722085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acid phosphatase I (AP-I) is the major isoform of Vigna acid phosphatase. It is constitutively expressed in seed cotyledons during germination. AP-I was separated from other isoforms and purified to homogeneity by three simple purification steps; (NH4)2SO4 precipitation, and phosphocellulose and DEAE-cellulose column chromatography. The activity of AP-I was not affected by 1 mM Mg2+, Mn2+, Ca2+, Co2+ or Pb2+, but severely inhibited by 1 mM Cu2+, Fe3+, Hg2+, Mo6+ or Zn2+. AP-I has both phosphatase and pyrophosphatase activities, and is highly stable even at 50 degrees.
Authors:
T K Biswas; M Promo; B Biswas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Phytochemistry     Volume:  41     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-02-12     Completed Date:  1997-02-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1457-8     Citation Subset:  B    
Affiliation:
Department of Pathology, University of Chicago, IL 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / isolation & purification*,  metabolism*
Ammonium Sulfate
Cations / pharmacology
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Fabaceae / enzymology*
Isoenzymes / isolation & purification*,  metabolism*
Kinetics
Plants, Medicinal*
Seeds
Grant Support
ID/Acronym/Agency:
GM 47428/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cations; 0/Isoenzymes; 7783-20-2/Ammonium Sulfate; EC 3.1.3.2/Acid Phosphatase

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