Document Detail

Purification of Toxoplasma gondii oocysts by cesium chloride gradient.
MedLine Citation:
PMID:  14967234     Owner:  NLM     Status:  MEDLINE    
We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a > 96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (< or = 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts.
Aurélien Dumètre; Marie-Laure Dardé
Related Documents :
11881924 - Offstream water and trace mineral salt as management strategies for improved cattle dis...
866124 - Chronic copper toxicosis in sheep.
24168694 - Gustatory cortical lesions affect motivation for snack foods.
24244 - Clinical and metabolic consequences to cattle of rumen overdosing with di-ureido iso-bu...
18938004 - Influence of toxic cyanobacteria on community structure and microcystin accumulation of...
17954734 - Profile of gelatinolytic capacity of raw goat milk and the implications for milk quality.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  56     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-17     Completed Date:  2004-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  427-30     Citation Subset:  IM    
UPRES EA 3174, Neuroparasitologie et neuroépidémiologie tropicale, Faculté de Médecine, 2 Rue du Dr Marcland, F-87025 Limoges, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Centrifugation, Density Gradient / methods*
Oocysts / immunology,  isolation & purification*,  metabolism
Time Factors
Toxoplasma / cytology,  growth & development,  isolation & purification*
Reg. No./Substance:
0/Chlorides; 7440-46-2/Cesium; 7647-17-8/cesium chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification of microorganisms by PCR amplification and sequencing of a universal amplified riboso...
Next Document:  A rapid DNA extraction method for PCR identification of fungal indoor air contaminants.