Document Detail


Purification of Toxoplasma gondii oocysts by cesium chloride gradient.
MedLine Citation:
PMID:  14967234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a > 96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (< or = 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts.
Authors:
Aurélien Dumètre; Marie-Laure Dardé
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  56     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-17     Completed Date:  2004-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  427-30     Citation Subset:  IM    
Affiliation:
UPRES EA 3174, Neuroparasitologie et neuroépidémiologie tropicale, Faculté de Médecine, 2 Rue du Dr Marcland, F-87025 Limoges, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Centrifugation, Density Gradient / methods*
Cesium
Chlorides
Oocysts / immunology,  isolation & purification*,  metabolism
Time Factors
Toxoplasma / cytology,  growth & development,  isolation & purification*
Chemical
Reg. No./Substance:
0/Chlorides; 7440-46-2/Cesium; 7647-17-8/cesium chloride

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