Document Detail


Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen.
MedLine Citation:
PMID:  2610679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequential chromatography on Phenyl-Sepharose, CM-Sepharose, concanavalin A-Sepharose, FPLC Superose-12 and FPLC Mono-S. The purification over the original tissue extract was 1150- and 3300-fold, with a yield of 2.1% and 2.5%, respectively. Gel filtration indicated an Mr of about 30000 for both forms. There was a N-terminal sequence identity between the two enzymes. However, they appeared to be different as assessed by cation exchange chromatography and amino acid composition.
Authors:
J J Stĕpán; K H Lau; S Mohan; M Kraenzlin; D J Baylink
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  165     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-13     Completed Date:  1990-02-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1027-34     Citation Subset:  IM    
Affiliation:
Department of Medicine, Loma Linda University, CA.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / isolation & purification*
Amino Acid Sequence
Amino Acids / analysis
Chromatography
Chromatography, High Pressure Liquid
Drug Resistance
Humans
Leukemia, Hairy Cell / enzymology*
Male
Middle Aged
Molecular Sequence Data
Molecular Weight
Spleen / enzymology*
Tartrates / pharmacology*
Grant Support
ID/Acronym/Agency:
AR31062/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Tartrates; 526-83-0/tartaric acid; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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