| Purification and identification of 1-deoxynojirimycin (DNJ) in okara fermented by Bacillus subtilis B2 from Chinese traditional food (Meitaoza). | |
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MedLine Citation:
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PMID: 20196601 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was to purify an alpha-glucosidase inhibitor from okara (soy pulp) fermented by Bacillus subtilis B2 and to identify its chemical structure. Membrane dialysis, active charcoal, CM-Sepharose chromatography, and preparative thin-layer chromatography (TLC) were used in the purification, while positive mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry were used in the identification. The MS and NMR data showed that the purified alpha-glucosidase inhibitor was 1-deoxynojirimycin (DNJ) with a molecular weight of 163 Da. This is the first time that DNJ was isolated from foods fermented with Bacillus species. Okara fermentation with B. subtilis B2 might be used to produce a food-derived DNJ product as a functional food for diabetic patients. |
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Authors:
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Yun-Ping Zhu; Kohji Yamaki; Tadashi Yoshihashi; Mayumi Ohnishi Kameyama; Xiu-Ting Li; Yong-Qiang Cheng; Yutaka Mori; Li-Te Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-08-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 4097-103 Citation Subset: IM |
Affiliation:
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Department of Food Science, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Haidian District, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Deoxynojirimycin
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chemistry,
isolation & purification*,
metabolism Bacillus subtilis / genetics, isolation & purification, metabolism* Enzyme Inhibitors / chemistry, isolation & purification, metabolism Fermentation* Food Microbiology* alpha-Glucosidases / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 19130-96-2/1-Deoxynojirimycin; EC 3.2.1.20/alpha-Glucosidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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