Document Detail


Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes.
MedLine Citation:
PMID:  23315653     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.
Authors:
Christopher Schirwitz; Felix F Loeffler; Thomas Felgenhauer; Volker Stadler; Alexander Nesterov-Mueller; Reiner Dahint; Frank Breitling; F Ralf Bischoff
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-14
Journal Detail:
Title:  Advanced materials (Deerfield Beach, Fla.)     Volume:  -     ISSN:  1521-4095     ISO Abbreviation:  Adv. Mater. Weinheim     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9885358     Medline TA:  Adv Mater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. c.schirwitz@dkfz.de.
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