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Purification of Extracellular Cholesterol Oxidase with High Activity in the Presence of Organic Solvents from Pseudomonas sp. Strain ST-200
MedLine Citation:
PMID:  9572974     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Extracellular cholesterol oxidase of Pseudomonas sp. strain ST-200 was purified from the culture supernatant. This oxidase contained bound flavin and was categorized as a 3beta-hydroxysteroid oxidase, converting 3beta-hydroxyl groups to keto groups. The molecular mass was 60 kDa. The enzyme was stable at pH 4 to 11 and active at pH 5.0 to 8.5, showing optimal activity at pH 7 at 60 degreesC. The Michaelis constant of the ST-200 cholesterol oxidase was lower than those of commercially available oxidases. The cholesterol oxidation rate was enhanced 3- to 3.5-fold in the presence of organic solvents, with log Pow values (partition coefficients of the organic solvent between n-octanol and water), in the range of 2.1 to 4.2, compared with that in the absence of organic solvents.
Authors:
Doukyu; Aono
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  64     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-05-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1929-32     Citation Subset:  -    
Affiliation:
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226, Japan.
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