| Purification and characterization of two extracellular xylanases from Penicillium sclerotiorum: a novel acidophilic xylanase. | |
| | |
MedLine Citation:
|
PMID: 19680819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two xylanases from the crude culture filtrate of Penicillium sclerotiorum were purified to homogeneity by a rapid and efficient procedure, using ion-exchange and molecular exclusion chromatography. Molecular masses estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were 23.9 and 33.1 kDa for xylanase I and II, respectively. The native enzymes' molecular masses of 23.8 and 30.8 kDa were estimated for xylanase I and II, respectively, by molecular exclusion chromatography. Both enzymes are glycoproteins with optimum temperature and pH of 50 degrees C and pH 2.5 for xylanase I and 55 degrees C and pH 4.5 for xylanase II. The reducing agents beta-mercaptoethanol and dithio-treitol enhanced xylanase activities, while the ions Hg(2+) and Cu(2+) as well the detergent SDS were strong inhibitors of both enzymes, but xylanase II was stimulated when incubated with Mn(2+). The K (m) value of xylanase I for birchwood xylan and for oat spelt xylan were 6.5 and 2.6 mg mL(-1), respectively, whereas the K (m) values of xylanase II for these substrates were 26.61 and 23.45 mg mL(-1). The hydrolysis of oat spelt xylan by xylanase I released xylobiose and larger xylooligosaccharides while xylooligosaccharides with a decreasing polymerization degree up to xylotriose were observed by the action of xylanase II. The present study is among the first works to examine and describe an extracellular, highly acidophilic xylanase, with an unusual optimum pH at 2.5. Previously, only one work described a xylanase with optimum pH 2.0. This novel xylanase showed interesting characteristics for biotechnological process such as feed and food industries. |
| | |
Authors:
|
Adriana Knob; Eleonora Cano Carmona |
Related Documents
:
|
20419509 - Production of xylanase from an alkali tolerant streptomyces sp. 7b under solid-state fe... 9571799 - Enzymatic specificity and hydrolysis pattern of the catalytic domain of the xylanase xy... 12680939 - Physical properties of an extracellular polysaccharide produced by bacillus sp. cp912. 18038909 - Purification and biochemical properties of phospholipase d (pld57) produced by streptom... 19054669 - Characterization of a hap-phytase from a thermophilic mould sporotrichum thermophile. 17920159 - Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in ... 6217929 - Impaired degradation of chondroitin sulfate in gm2-gangliosidosis. 8818219 - Products of s cerevisiae cis-prenyltransferase activity in vitro. 8654569 - Reoxidation of the nadph produced by the pentose phosphate pathway is necessary for the... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-13 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: 162 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-05-04 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 429-43 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Microbiology, São Paulo State University, Avenue 24-A, 1515, 13506-900 Rio Claro, São Paulo, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Gel Enzyme Stability Extracellular Space / enzymology Hydrogen-Ion Concentration Molecular Weight Penicillium / enzymology* Substrate Specificity Temperature Xylosidases / isolation & purification*, metabolism* |
| Chemical | |
Reg. No./Substance:
|
EC 3.2.1.-/Xylosidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Utilization of Gum Tragacanth to Improve the Growth of Rhodotorula aurantiaca and the Production...
Next Document: Treatment with Tertiary Oximes Prevents Seizures and Improves Survival Following Sarin Intoxication.