| Purification and Characterization of Polyphenols from Chestnut Astringent Skin. | |
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MedLine Citation:
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PMID: 21777007 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Polyphenolic compounds from chestnut astringent skin (CAS) were purified by dialysis, using Diaion HP-20 and Sephadex LH-20 columns. During purification, specific α-amylase inhibitory activities were increased about 3.4-fold and the 50% inhibition value was 5.71 μg/mL in the Sephadex LH-20 fraction (SE-fraction). The SE-fraction contained about 67 % of the total polyphenols, 57.3% of the flavanol-type tannins and 51.3 % of the procyanidins. Strong antioxidant activity was observed in the SE-fraction. Oral administration of the SE-fraction in rats fed corn starch significantly suppressed an increase in blood glucose levels. The SE-fraction contained gallic acid and ellagic acid. The MALDI-TOF spectrum showed a peak series exhibiting a mass increment of 288 Da, reflecting the variation in the number of catechin/epicatechin units. Our results suggest CAS contains polyphenols with strong α-amylase inhibitory activity. The data also suggests CAS polyphenols might be oligomeric proanthocyanidins with gallic acid and ellagic acid. |
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Authors:
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Tsujita Takahiro; Misato Yamada; Takeshi Takaku; Tomoyashi Shintani; Kanae Teramoto; Takafumi Sato |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-21 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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