Document Detail


Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase.
MedLine Citation:
PMID:  23150407     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bacterial virulence proteins often mimic host eukaryotic proteins to modify or disturb host cellular -pathways. Increasing lines of evidence show that many bacterial effector proteins have E3 ubiquitin ligase activity. The effector protein LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes, ubiquitin ligase activity is analyzed by the in vitro ubiquitination assay.
Authors:
Hiroki Nagai; Tomoko Kubori
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  347-354     Citation Subset:  -    
Affiliation:
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan, hnagai@biken.osaka-u.ac.jp.
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