| Purification and characterization of caprine ghrelin and its effect on growth hormone release. | |
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MedLine Citation:
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PMID: 20437258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ghrelin, a novel peptide modified by n-octanoic acid at the third serine residue (Ser(3)), serves as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R) 1a. The octanoyl modification at Ser(3) is essential for receptor binding or growth hormone release. Here, we report the purification of caprine ghrelin and its physiological role in goats. The major form of caprine ghrelin is a 27 amino acid peptide that is octanoylated (C8:0) at Ser(3) and lacks Gln(14), which is present in rat and human ghrelin. Additionally, we identified various acyl modifications in caprine ghrelin: nonanoic (C9:0), decanoic (10:0), unsaturated octanoic acids (C8:1), and an unidentified fatty acid modification. We observed that differences in acyl modifications affected GHS-R1a activation. In addition, administration of synthetic bovine ghrelin increased plasma growth hormone (GH) levels in goats. Thus, the present study indicates a structural divergence in caprine ghrelin and suggests that ghrelin is involved in GH release in ruminants. |
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Authors:
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Takanori Ida; Mikiya Miyazato; Xing-Zi Lin; Hiroyuki Kaiya; Takahiro Sato; Keiko Nakahara; Noboru Murakami; Kenji Kangawa; Masayasu Kojima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-02 |
Journal Detail:
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Title: Journal of molecular neuroscience : MN Volume: 42 ISSN: 1559-1166 ISO Abbreviation: J. Mol. Neurosci. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002991 Medline TA: J Mol Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 99-105 Citation Subset: IM |
Affiliation:
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Interdisciplinary Research Organization, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. a0d203u@cc.miyazaki-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular Fatty Acids / chemistry Ghrelin / chemistry, genetics, isolation & purification*, metabolism* Goats* Growth Hormone / secretion* Humans Male Molecular Sequence Data Rats Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Ghrelin; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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