Document Detail


Purification and Biochemical Characterization of a Highly Thermostable Bacteriocin Isolated from Brevibacillus brevis Strain GM100.
MedLine Citation:
PMID:  23291759     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A bacteriocin-producing (11,000 AU mL(-1)) strain was isolated from the rhizosphere of healthy Algerian plants Ononis angustissima Lam., and identified as Brevibacillus brevis strain GM100. The bacteriocin, called Bac-GM100, was purified to homogeneity from the culture supernatant, and, based on MALDI-TOF/MS analysis, was a monomer protein with a molecular mass of 4375.66 Da. The 21 N-terminal residues of Bac-GM100 displayed 65% homology with thurincin H from Bacillus thuringiensis. Bac-GM100 was extremely heat-stable (20 min at 120 °C), and was stable within a pH range of 3-10. It proved sensitive to various proteases, which demonstrated its protein nature. It was also found to display a bactericidal mode of action against gram-negative (Salmonella enteric ATCC 43972, Pseudomonas aeruginosa ATCC 49189, and Agrobacterium tumefaciens C58) and gram-positive (Enterococcus faecalis ENSAIA 631 and Staphylococcus aureus ATCC 6538) bacteria, and a fungistatic mode of action against the pathogenic fungus Candida tropicalis R2 CIP 203.
Authors:
Mouloud Ghadbane; Daoud Harzallah; Atef Ibn Laribi; Bassem Jaouadi; Hani Belhadj
Related Documents :
23569909 - Additive potential of ginger starch on antifungal potency of honey against candida albi...
23231489 - Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea.
23056609 - Mechanism of action of recombinant acc-royalisin from royal jelly of asian honeybee aga...
23162679 - The discovery of n-1 substituted 2-aminobenzimidazoles as zinc-dependent s. aureus biof...
14100969 - Electrical excitability of isolated frog skin and toad bladder.
23019189 - Antibacterial mechanisms of rhodomyrtone against important hospital-acquired antibiotic...
8896519 - Patient isolates of borrelia burgdorferi sensu lato with genotypic and phenotypic simil...
9427459 - Increased prevalence of penicillin-resistant viridans group streptococci in japanese ch...
11321089 - Taxonomic study of aerobic thermophilic bacilli: descriptions of geobacillus subterrane...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  -     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bi-Phasic Effect of Equol on Adipocyte Differentiation of MC3T3-L1 Cells.
Next Document:  Isolation and Characterization of an Anti-Insect ?-Toxin from the Venom of the Scorpion Isometrus ma...