Document Detail


Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus.
MedLine Citation:
PMID:  7751356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriophage typing (BT) (World Health Organization method) has been used at the Centers for Disease Control and Prevention for over 30 years to type isolates of Staphylococcus aureus. Since studies have shown that BT patterns have poor reproducibility and because BT fails to type a high percentage (15 to 20%) of isolates, the Centers for Disease Control and Prevention has converted from using BT to using pulsed-field gel electrophoresis (PFGE) for strain typing S. aureus. We compared the results of BT with results of PFGE for typing 300 isolates of S. aureus, including strains from several well-characterized outbreaks. Ninety-six isolates were BT group I, 19 were group II, 82 were group III, 7 were group V, and 96 were nontypeable. PFGE identified subgroups within each phage group and thus was more discriminating than BT, which identified no subgroups. PFGE was able to type all isolates and distinguish related from unrelated strains of S. aureus. Our modified, standardized PFGE methodology should enable typing laboratories to obtain rapid, reliable results in 3 to 4 days when starting with an isolated colony on agar media.
Authors:
T L Bannerman; G A Hancock; F C Tenover; J M Miller
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  33     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  551-5     Citation Subset:  IM    
Affiliation:
National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage Typing / methods*
Electrophoresis, Gel, Pulsed-Field*
Predictive Value of Tests
Reproducibility of Results
Staphylococcus Phages
Staphylococcus aureus / classification*
Comments/Corrections

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