Document Detail


Pulsed electric fields inactivation of attached and free-living Escherichia coli and Listeria innocua under several conditions.
MedLine Citation:
PMID:  10746578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of pulsed electric fields (PEF) is considered as a mild process in the inactivation of microorganisms present in liquid food products. PEF treatments of Escherichia coli and Listeria innocua suspended in milk and phosphate buffer, with same pH and same conductivities, yielded to similar inactivation. Reduction rates obtained in distilled water indicated that conductivity of the food product is a main parameter in bacterial inactivation. Bacteria attached to polystyrene beads were inactivated by PEF at a greater (E. coli) or equal rate (L. innocua) than free-living bacteria. Base on the use of selective and non-selective enumeration media, no clear indications were obtained for sublethal damage of microorganisms surviving the PEF treatment. E. coli cells subjected to 60 pulses at 41 kV/cm were examined by transmission and scanning electron microscopy. Changes in the cytoplasm were observed and the cell surface appeared rough. The cells outer membranes were partially destroyed allowing leaking of cell cytoplasm.
Authors:
N Dutreux; S Notermans; T Wijtzes; M M Góngora-Nieto; G V Barbosa-Cánovas; B G Swanson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  54     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-03     Completed Date:  2000-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  IM    
Affiliation:
TNO Nutrition and Food Research Institute, Zeist, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Electricity
Escherichia coli* / ultrastructure
Food Microbiology*
Listeria* / ultrastructure
Microscopy, Electron
Pulsatile Flow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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