Document Detail

Pulse radiolysis study of a yeast cytochrome c from Hansenula anomala.
MedLine Citation:
PMID:  3015259     Owner:  NLM     Status:  MEDLINE    
The reduction of Hansenula anomala yeast cytochrome c by e-aq and CO-.2 was investigated by pulse radiolysis, at a high reductant to protein concentration ratio. The reactivity of the radicals was studied by observing absorbance changes in the cytochrome c spectrum over the wavelength range 280-600 nm. At pH 7, over the time scale of the radical decays (i.e. 0-4 microseconds for e-aq; 0-40 microseconds for CO-.2s) and beyond, the hemoprotein was reduced without any spectrally detected intermediate between ferri-and ferro-forms. This conclusion was reached by simulation studies based on the direct reduction of the yeast cytochrome c from the ferri- to the ferro-form, yielding a correct fit between experimental and calculated absorbance curves. The reduction rate constants were determined to be 1.0 +/- 01 X 10(10) M-1 S-1 for e-aq and 0.7 +/- 0.05 X 10(9) M-1 S-1 for CO-.2 at 0.16 M ionic strength, pH 7.0 and 20 degrees C, thus not significantly different from other values reported for horse heart cytochrome c. However, in the 360-390 nm region the generation of an additional radical species was noticed. The present experimental data were compared with previously published reports.
M A Thomas; C Capellere-Blandin; J Pucheault; C Ferradini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  68     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-09-16     Completed Date:  1986-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  745-55     Citation Subset:  IM    
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MeSH Terms
Carbon Dioxide / metabolism
Cytochrome c Group / metabolism*
Free Radicals
Pichia / analysis*
Pulse Radiolysis
Saccharomycetales / analysis*
Reg. No./Substance:
0/Cytochrome c Group; 0/Free Radicals; 0/Solutions; 124-38-9/Carbon Dioxide; 14485-07-5/carboxyl radical; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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