Document Detail


Pulse cytophotometric analysis of synchronized cells in vitro.
MedLine Citation:
PMID:  130204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulse cytophotometry is a reliable rapid technique rendering a detailed direct analysis of the distribution of cells in G1/10, S, and (G2 + M) phase. We used a Phywe pulse cytophotometer ICP 11 to monitor cell cycle progression of synchronized human lymphoma cells in culture. With mithramycin as the fluorescent dye, sample processing is fast and provides DNA histograms of high resolution and precision. Results obtained from these histograms are in excellent agreement with those obtained by conventional techniques. Thus, we have established the conditions necessary to apply pulse cytophotometry for studies of drug-induced cytokinetic effects on this cell line.
Authors:
B Barlogie; B Drewinko; D A Johnston; T Büchner; W H Hauss; E J Freireich
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  36     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-06-02     Completed Date:  1976-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1176-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cell Line
Cells, Cultured
Cytological Techniques*
Lymphoma / pathology
Plicamycin
Chemical
Reg. No./Substance:
18378-89-7/Plicamycin

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