| Pulse cytophotometric analysis of synchronized cells in vitro. | |
MedLine Citation:
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PMID: 130204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pulse cytophotometry is a reliable rapid technique rendering a detailed direct analysis of the distribution of cells in G1/10, S, and (G2 + M) phase. We used a Phywe pulse cytophotometer ICP 11 to monitor cell cycle progression of synchronized human lymphoma cells in culture. With mithramycin as the fluorescent dye, sample processing is fast and provides DNA histograms of high resolution and precision. Results obtained from these histograms are in excellent agreement with those obtained by conventional techniques. Thus, we have established the conditions necessary to apply pulse cytophotometry for studies of drug-induced cytokinetic effects on this cell line. |
Authors:
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B Barlogie; B Drewinko; D A Johnston; T Büchner; W H Hauss; E J Freireich |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 36 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1976 Mar |
Date Detail:
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Created Date: 1976-06-02 Completed Date: 1976-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1176-81 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division Cell Line Cells, Cultured Cytological Techniques* Lymphoma / pathology Plicamycin |
| Chemical | |
Reg. No./Substance:
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18378-89-7/Plicamycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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