| Pulmonary surfactant protein SP-B is significantly more immunoreactive in anionic than in zwitterionic bilayers. | |
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MedLine Citation:
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PMID: 11311247 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Binding of polyclonal and monoclonal antibodies, quantitated by enzyme-linked immunosorbent assay, to porcine SP-B reconstituted in different phospholipid bilayers has been used to assess differences in protein structure due to lipid-protein interactions. SP-B bound significantly more antibodies when it was reconstituted in bilayers made of anionic phospholipids (phosphatidic acid, cardiolipin, phosphatidylglycerol, phosphatidylinositol or phosphatidylserine) than in zwitterionic bilayers (phosphatidylcholine, phosphatidylcholine/cholesterol, or phosphatidylethanolamine) or in fatty acid micelles (made of salts of palmitic or stearic acids). These differences in immunoreactivity can be important in the development of quantitation methods for SP-B in clinical samples based on immunological techniques. |
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Authors:
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J M Oviedo; C Casals; J Pérez-Gil |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 494 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-20 Completed Date: 2001-05-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 236-40 Citation Subset: IM |
Affiliation:
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Departamento Bioquímica y Biología Molecular I, Facultad Biología, Universidad Complutense, 28040 Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anions / chemistry, metabolism Antibodies, Monoclonal / immunology Antigens / immunology*, metabolism* Enzyme-Linked Immunosorbent Assay Immune Sera / immunology Lipid Bilayers / chemistry*, metabolism* Micelles Palmitic Acid / metabolism Phospholipids / chemistry, metabolism Proteolipids / immunology*, metabolism* Pulmonary Surfactants / immunology*, metabolism* Static Electricity Stearic Acids / metabolism Swine |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Antibodies, Monoclonal; 0/Antigens; 0/Immune Sera; 0/Lipid Bilayers; 0/Micelles; 0/Phospholipids; 0/Proteolipids; 0/Pulmonary Surfactants; 0/Stearic Acids; 57-10-3/Palmitic Acid; 57-11-4/stearic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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