Document Detail

Pulmonary surfactant disaturated-phosphatidylcholine (DSPC) turnover and pool size in newborn infants with congenital diaphragmatic hernia (CDH).
MedLine Citation:
PMID:  12904604     Owner:  NLM     Status:  MEDLINE    
In animal CDH models, surfactant deficiency contributes to the pathophysiology of the condition but information on human disease is very limited. The aim of our study was to investigate surfactant kinetics in CDH newborns. We studied surfactant disaturated-phosphatidylcholine (DSPC) half-life, turnover and apparent pool size by stable isotope methodology in CDH newborns with no ExtraCorporeal Membrane Oxygenation (ECMO) support (n = 13, birth weight (BW) 3.2 +/- 2.2 kg, gestational age (GA) 39 +/- 0.4 wks, postnatal age 43 +/- 11 h) and in 8 term infants with no lung disease (CONTROLS, BW 2.7 +/- 0 kg, GA 38 +/- 0.8 wks, postnatal age 96 +/- 26 h). We administered a trace dose of 13C-palmitic acid dipalmitoyl-phosphatidylcholine (DPPC) through the endotracheal (ET) tube and we measured DSPC kinetics by gas chromatography-mass spectrometry from DSPC13C-enrichment decay curves obtained from sequential tracheal aspirates. DSPC amount from tracheal aspirates (TA-DSPC) was measured by gas chromatography. In CDH infants DSPC half-life was shorter (24 +/- 4 and 53 +/- 11 h, p = 0.01), turnover faster (0.6 +/- 0.1 and 1.5 +/- 0.3 d-1 p = 0.01), apparent pool size smaller (34 +/- 6 and 57 +/- 7 mg/kg body weight, p = 0.02) and tracheal aspirates DSPC amount lower (2.4 +/- 0.4 and 4.6 +/- 0.5 mg/mL Epithelial Lining Fluid (ELF), p = 0.007) than in CONTROLS. In conclusion surfactant kinetics is grossly abnormal in mechanically ventilated CDH. Whether alterations of DSPC kinetics in CDH infants are caused by a primary surfactant deficiency or are secondary to oxygen therapy and ventilator support has still to be determined.
Paola E Cogo; Luc J I Zimmermann; Luisa Meneghini; Nicoletta Mainini; Linda Bordignon; Vincenzo Suma; Marika Buffo; Virgilio P Carnielli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-06
Journal Detail:
Title:  Pediatric research     Volume:  54     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-27     Completed Date:  2004-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  653-8     Citation Subset:  IM    
Department of Pediatrics, University of Padova, Padova, Italy.
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MeSH Terms
Extracorporeal Membrane Oxygenation
Gestational Age
Hernia, Diaphragmatic / congenital*
Infant, Newborn
Lung Compliance
Phosphatidylcholines / metabolism*
Pulmonary Surfactants / chemistry*,  metabolism*
Radioactive Tracers
Respiration, Artificial / adverse effects*
Reg. No./Substance:
0/Phosphatidylcholines; 0/Pulmonary Surfactants; 0/Radioactive Tracers; 0/lecithins, disaturated

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