Document Detail

Pulmonary neuroendocrine cells in cultures of human infant airway mucosa from postmortem tissues.
MedLine Citation:
PMID:  8703479     Owner:  NLM     Status:  MEDLINE    
Airway mucosa was isolated by enzymatic dissociation from tracheas and bronchi obtained at autopsy from stillbirth, infants, and children. An epithelial cell fraction was recovered that could be cultured on a collagen-coated substratum for at least 7 days. Epithelial cells were identified immunocytochemically with anticytokeratin antibody; pulmonary neuroendocrine cells (PNEC) were identified with antibodies against serotonin, bombesin/GRP, and MOC-1 cell surface antigen. Transmission electron microscopy confirmed neuroendocrine ultrastructural features of PNEC including the presence of neurosecretory dense core vesicles. The ability to recover and maintain viable PNEC in human lung airway epithelial cell cultures from postmortem tissues should facilitate further investigations of PNEC function in normal human lung and in various disease states.
H Yeger; V Speirs; C Youngson; E Cutz
Related Documents :
797569 - Isolation of cells that retain differentiated functions in vitro: properties of clonall...
3718999 - Metabolic and ultrastructural characterization of guinea pig alveolar type ii cells iso...
11002389 - Biochemical and morphological changes in lung tissue and isolated lung cells of rats in...
1489139 - Distribution of lung cell numbers and volumes between alveolar and nonalveolar tissue.
2072409 - Different metastasis patterns of a human melanoma cell line in nude mice and rats: infl...
9389569 - Differential expression and biological activity of the heparin-binding growth-associate...
15306019 - Defective cell wall synthesis in streptococcus pneumoniae r6 depleted for the essential...
8092819 - Intracellular metabolism of (-)- and (+)-cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiol...
8187649 - Basic fibroblast growth factor promotes adhesive interactions of neuroepithelial cells ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  15     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-10     Completed Date:  1996-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  232-6     Citation Subset:  IM    
Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bronchi / cytology*
Cell Culture Techniques / methods*
Cell Division / physiology
Cilia / ultrastructure
Epithelial Cells
Epithelium / ultrastructure
Fetal Death
Infant, Newborn
Microscopy, Electron
Mucous Membrane / cytology
Neurosecretory Systems / cytology*
Trachea / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Neutrophil transepithelial migration is dependent upon epithelial characteristics.
Next Document:  Expression of MUC1 mucin gene by hamster tracheal surface epithelial cells in primary culture.