| Pulmonary neuroendocrine cells in cultures of human infant airway mucosa from postmortem tissues. | |
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MedLine Citation:
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PMID: 8703479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Airway mucosa was isolated by enzymatic dissociation from tracheas and bronchi obtained at autopsy from stillbirth, infants, and children. An epithelial cell fraction was recovered that could be cultured on a collagen-coated substratum for at least 7 days. Epithelial cells were identified immunocytochemically with anticytokeratin antibody; pulmonary neuroendocrine cells (PNEC) were identified with antibodies against serotonin, bombesin/GRP, and MOC-1 cell surface antigen. Transmission electron microscopy confirmed neuroendocrine ultrastructural features of PNEC including the presence of neurosecretory dense core vesicles. The ability to recover and maintain viable PNEC in human lung airway epithelial cell cultures from postmortem tissues should facilitate further investigations of PNEC function in normal human lung and in various disease states. |
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Authors:
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H Yeger; V Speirs; C Youngson; E Cutz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of respiratory cell and molecular biology Volume: 15 ISSN: 1044-1549 ISO Abbreviation: Am. J. Respir. Cell Mol. Biol. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-09-10 Completed Date: 1996-09-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 232-6 Citation Subset: IM |
Affiliation:
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Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autopsy Bronchi / cytology* Cell Culture Techniques / methods* Cell Division / physiology Cilia / ultrastructure Epithelial Cells Epithelium / ultrastructure Fetal Death Humans Immunohistochemistry Infant Infant, Newborn Microscopy, Electron Mucous Membrane / cytology Neurosecretory Systems / cytology* Trachea / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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