Document Detail

Pulmonary bacterial communities in surgically resected noncystic fibrosis bronchiectasis lungs are similar to those in cystic fibrosis.
MedLine Citation:
PMID:  22448327     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Background. Recurrent bacterial infections play a key role in the pathogenesis of bronchiectasis, but conventional microbiologic methods may fail to identify pathogens in many cases. We characterized and compared the pulmonary bacterial communities of cystic fibrosis (CF) and non-CF bronchiectasis patients using a culture-independent molecular approach. Methods. Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. Results. A wide range of bacterial diversity was detected in both groups, with between 1 and 21 bacterial taxa found in each patient. Pseudomonas was the most common genus in both groups, comprising 49% of sequences detected and dominating numerically in 13 patients. Although Pseudomonas appeared to be dominant more often in CF patients than in non-CF patients, analysis of entire bacterial communities did not identify significant differences between these two groups. Conclusions. Our data indicate significant diversity in the pulmonary bacterial community of both CF and non-CF bronchiectasis patients and suggest that this community is similar in surgically resected lungs of CF and non-CF bronchiectasis patients.
Heather Maughan; Kristopher S Cunningham; Pauline W Wang; Yu Zhang; Marcelo Cypel; Cecilia Chaparro; D Elizabeth Tullis; Thomas K Waddell; Shaf Keshavjee; Mingyao Liu; David S Guttman; David M Hwang
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Publication Detail:
Type:  Journal Article     Date:  2012-02-08
Journal Detail:
Title:  Pulmonary medicine     Volume:  2012     ISSN:  2090-1844     ISO Abbreviation:  Pulm Med     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-26     Completed Date:  2012-08-23     Revised Date:  2013-02-27    
Medline Journal Info:
Nlm Unique ID:  101558762     Medline TA:  Pulm Med     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  746358     Citation Subset:  -    
Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, Canada M5S 3B2.
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