Document Detail


Pulmonary allergic reactions impair systemic vascular relaxation in ragweed sensitive mice.
MedLine Citation:
PMID:  20888432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asthma is often associated with cardiovascular complications, and recent observations in animal models indicate that induction of pulmonary allergic inflammation increases susceptibility of the myocardium to ischemia and reperfusion injury. In this study, we used a murine model of allergen sensitization in which aspiration of allergen induces pulmonary and systemic inflammation, to test the hypothesis that pulmonary exposure to allergen alters vascular relaxation responses. BALB/C mice were sensitized by intraperitoneal injection of ragweed and challenged by intratracheal instillation of allergen. Airway hyperreactivity and pulmonary inflammation were confirmed, and endothelium-dependent and -independent reactivity of thoracic aorta rings were evaluated. Ragweed sensitization and challenge induced airway hyperreactivity to methacholine and pulmonary inflammation, but did not affect constrictor responses of the aortic rings to phenylephrine and K+ depolarization. In contrast, maximal relaxation of aortic rings to acetylcholine and sodium nitroprusside decreased from 87.6±3.9% and 97.7±1.2% to 32±4% and 51±6%, respectively (p<0.05). The sensitivity to acetylcholine was likewise reduced (EC₅₀=0.26±0.05 μM vs. 1.09±0.16 μM, p<0.001). The results demonstrate that induction of allergic pulmonary inflammation in mice depresses endothelium-dependent and -independent vascular relaxation, which can contribute to cardiovascular complications associated with allergic inflammation.
Authors:
Surovi Hazarika; Michael R Van Scott; Robert M Lust; Christopher J Wingard
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-01
Journal Detail:
Title:  Vascular pharmacology     Volume:  53     ISSN:  1879-3649     ISO Abbreviation:  Vascul. Pharmacol.     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-11-15     Completed Date:  2011-03-28     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101130615     Medline TA:  Vascul Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  258-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Ambrosia / immunology*
Animals
Aorta, Thoracic / immunology,  physiopathology
Asthma / immunology,  physiopathology*
Bronchial Hyperreactivity / immunology,  physiopathology*
Bronchoalveolar Lavage Fluid / immunology
Dose-Response Relationship, Drug
Inflammation / immunology,  physiopathology
Lung / immunology,  physiopathology
Male
Methacholine Chloride / immunology
Mice
Mice, Inbred BALB C
Respiratory Hypersensitivity / immunology*
Vasodilation / immunology*
Grant Support
ID/Acronym/Agency:
R01 ES016246/ES/NIEHS NIH HHS; R01 ES016246-01/ES/NIEHS NIH HHS; R01ES5016246/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Allergens; 62-51-1/Methacholine Chloride
Comments/Corrections

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