| The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. | |
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MedLine Citation:
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PMID: 20197405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Translation regulation plays an important role during gametocytogenesis in the malaria parasite, a process that is obligatory for the transmission of the parasite through mosquito vectors. In this study we determined the function of PfPuf2, a member of the Puf family of translational repressors, in gametocytogenesis of Plasmodium falciparum. Tagging of the endogenous PfPuf2 protein with green fluorescent protein showed that PfPuf2 was expressed in both male and female gametocytes, and the protein was localized in the cytoplasm of the parasite. Targeted disruption of the PfPuf2 gene did not affect asexual growth of the parasite, but promoted the formation of gametocytes and differentiation of male gametocytes. Complementation studies were performed to confirm that the resultant phenotypic changes were due to disruption of the PfPuf2 gene. Episomal expression of PfPuf2 under its cognate promoter almost restored the gametocytogenesis rate in a PfPuf2 disruptant to the level of the wild-type parasite. It also partially restored the effect of PfPuf2 disruption on male-female sex ratio. In addition, episomal overexpression of PfPuf2 under its cognate promoter but with a higher concentration of the selection drug or under the constitutive hsp86 promoter in both the PfPuf2-disruptant and wild-type 3D7 lines, further dramatically reduced gametocytogenesis rates and sex ratios. These findings suggest that in this early branch of eukaryotes the function of PfPuf2 is consistent with the ancestral function of suppressing differentiation proposed for Puf-family proteins. |
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Authors:
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Jun Miao; Jinfang Li; Qi Fan; Xiaolian Li; Xinyi Li; Liwang Cui |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-03-02 |
Journal Detail:
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Title: Journal of cell science Volume: 123 ISSN: 1477-9137 ISO Abbreviation: J. Cell. Sci. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-24 Completed Date: 2010-09-07 Revised Date: 2011-07-27 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 1039-49 Citation Subset: IM |
Affiliation:
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Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / genetics Cell Line Cloning, Molecular Cytoplasm Female Gametogenesis / genetics Germ Cells / metabolism* Malaria / genetics, parasitology* Male Plasmodium falciparum / genetics, growth & development, metabolism Protozoan Proteins / genetics, metabolism* RNA, Small Interfering / genetics RNA-Binding Proteins / genetics, metabolism* Saccharomyces cerevisiae Proteins / genetics Sex Differentiation* Sex Ratio |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI064553/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/PUF2 protein, S cerevisiae; 0/Protozoan Proteins; 0/RNA, Small Interfering; 0/RNA-Binding Proteins; 0/Saccharomyces cerevisiae Proteins |
| Comments/Corrections | |
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