Document Detail

Puerarin stimulates osteoblasts differentiation and bone formation through estrogen receptor, p38 MAPK, and Wnt/β-catenin pathways.
MedLine Citation:
PMID:  22917468     Owner:  NLM     Status:  MEDLINE    
Puerarin is an isoflavone extracted from Radix Puerariae, a traditional Chinese herb used to treat many diseases such as osteoporosis. In this study, puerarin was shown to stimulate alkaline phosphatase (ALP) activity, type I collagen (Col I) secretion, and mineralized nodules formation of primary osteoblasts. Whereas the estrogen receptor (ER) antagonist ICI 182780 was able to reduce the increase in ALP activity and Col I secretion induced by puerarin. Furthermore, puerarin was shown to elevate levels of phospho-p38 mitogen-activated protein kinase (MAPK) and β-catenin proteins in a time-dependent manner. Pretreatment of osteoblasts with ICI 182780 can reduce this elevation, whereas pretreatment with p38 MAPK inhibitor SB 203580 did not affect the increase of β-catenin protein. Meanwhile, intragastric administration of puerarin protected against reduction in bone mineral density and bone mineral content in ovariectomized rats, and improved femur trabecular bone structure. Taken together, ER, p38 MAPK, and Wnt/β-catenin pathways were involved in puerarin-stimulated osteoblasts differentiation and bone formation.
Pan-Pan Wang; Xiao-Feng Zhu; Li Yang; Heng Liang; Shui-Wang Feng; Rong-Hua Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-23
Journal Detail:
Title:  Journal of Asian natural products research     Volume:  14     ISSN:  1477-2213     ISO Abbreviation:  J Asian Nat Prod Res     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-18     Completed Date:  2012-12-19     Revised Date:  2013-04-03    
Medline Journal Info:
Nlm Unique ID:  100888334     Medline TA:  J Asian Nat Prod Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  897-905     Citation Subset:  IM    
Medical College of Jinan University, Guangzhou, China.
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MeSH Terms
Alkaline Phosphatase / metabolism*
Bone Density / drug effects
Cell Differentiation
Collagen Type I / drug effects,  metabolism*
Estradiol / chemistry,  pharmacology
Isoflavones / chemistry,  pharmacology*
Osteoblasts / drug effects,  metabolism*
Osteogenesis / drug effects*
Receptors, Estrogen / drug effects*
beta Catenin / drug effects*,  metabolism*
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors*,  metabolism
Reg. No./Substance:
0/Collagen Type I; 0/Isoflavones; 0/Receptors, Estrogen; 0/beta Catenin; 50-28-2/Estradiol; EC Mitogen-Activated Protein Kinases; EC Phosphatase; Z9W8997416/puerarin

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