Document Detail

Pseudorabies virus Us9 directs axonal sorting of viral capsids.
MedLine Citation:
PMID:  17686845     Owner:  NLM     Status:  MEDLINE    
Pseudorabies virus (PRV) mutants lacking the Us9 gene cannot spread from presynaptic to postsynaptic neurons in the rat visual system, although retrograde spread remains unaffected. We sought to recapitulate these findings in vitro using the isolator chamber system developed in our lab for analysis of the transneuronal spread of infection. The wild-type PRV Becker strain spreads efficiently to postsynaptic neurons in vitro, whereas the Us9-null strain does not. As determined by indirect immunofluorescence, the axons of Us9-null infected neurons do not contain the glycoproteins gB and gE, suggesting that their axonal sorting is dependent on Us9. Importantly, we failed to detect viral capsids in the axons of Us9-null infected neurons. We confirmed this observation by using three different techniques: by direct fluorescence of green fluorescent protein-tagged capsids; by transmission electron microscopy; and by live-cell imaging in cultured, sympathetic neurons. This finding has broad impact on two competing models for how virus particles are trafficked inside axons during anterograde transport and redefines a role for Us9 in viral sorting and transport.
M G Lyman; B Feierbach; D Curanovic; M Bisher; L W Enquist
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-08-08
Journal Detail:
Title:  Journal of virology     Volume:  81     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-27     Completed Date:  2008-01-08     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11363-71     Citation Subset:  IM    
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
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MeSH Terms
Axons / virology*
Biological Transport
Capsid / metabolism*
Capsid Proteins
Herpesvirus 1, Suid / pathogenicity*
Lipoproteins / physiology*
Neurons / virology*
Phosphoproteins / physiology*
Synapses / virology
Viral Proteins / physiology*
Grant Support
R01 33063//PHS HHS; R01 33506//PHS HHS
Reg. No./Substance:
0/Capsid Proteins; 0/Lipoproteins; 0/Phosphoproteins; 0/US9 protein, Suid herpesvirus 1; 0/Viral Proteins

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