Document Detail

The Pseudomonas putida Crc global regulator is an RNA binding protein that inhibits translation of the AlkS transcriptional regulator.
MedLine Citation:
PMID:  17462015     Owner:  NLM     Status:  MEDLINE    
The Crc protein is a global regulator that controls the hierarchical assimilation of carbon sources in Pseudomonads by inhibiting expression of several catabolic pathways. Crc does not bind DNA and its mechanism of action has remained elusive. Among other genes, Crc inhibits expression of alkS, the transcriptional activator of the Pseudomonas putida OCT plasmid alkane degradation pathway. AlkS activates expression of its own gene. In the presence of saturating AlkS levels, translational fusions of alkS to the lacZ reporter gene were responsive to Crc, but transcriptional fusions were not. In translational fusions, the first 33 nt of alkS mRNA, which includes up to position +3 relative to the translation start site, were sufficient to confer an efficient response to Crc. In vitro, purified Crc could bind specifically to an alkS mRNA fragment spanning positions +1 to +43, comprising the translation initiation region. We have previously shown that Crc has little effect on the stability of alkS mRNA. We conclude that Crc modulates AlkS levels by binding to the translation initiation region of alkS mRNA, thereby inhibiting translation. Because AlkS is an unstable protein present in limiting amounts, reducing its levels leads to decreased expression of all genes in the pathway.
Renata Moreno; Ana Ruiz-Manzano; Luis Yuste; Fernando Rojo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  64     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-27     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  665-75     Citation Subset:  IM    
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism*
Gene Expression Regulation, Bacterial
Lac Operon / genetics
Models, Biological
Models, Genetic
Operon / genetics
Protein Binding
Protein Biosynthesis / genetics*
Pseudomonas putida / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
RNA-Binding Proteins / genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Repressor Proteins / genetics,  metabolism*
Transcription, Genetic / genetics
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Messenger; 0/RNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/Repressor Proteins; 0/crc protein, Pseudomonas

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