Document Detail


The Pseudomonas putida Crc global regulator controls the expression of genes from several chromosomal catabolic pathways for aromatic compounds.
MedLine Citation:
PMID:  14973036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Crc protein is involved in the repression of several catabolic pathways for the assimilation of some sugars, nitrogenated compounds, and hydrocarbons in Pseudomonas putida and Pseudomonas aeruginosa when other preferred carbon sources are present in the culture medium (catabolic repression). Crc appears to be a component of a signal transduction pathway modulating carbon metabolism in pseudomonads, although its mode of action is unknown. To better understand the role of Crc, the proteome profile of two otherwise isogenic P. putida strains containing either a wild-type or an inactivated crc allele was compared. The results showed that Crc is involved in the catabolic repression of the hpd and hmgA genes from the homogentisate pathway, one of the central catabolic pathways for aromatic compounds that is used to assimilate intermediates derived from the oxidation of phenylalanine, tyrosine, and several aromatic hydrocarbons. This led us to analyze whether Crc also regulates the expression of the other central catabolic pathways for aromatic compounds present in P. putida. It was found that genes required to assimilate benzoate through the catechol pathway (benA and catBCA) and 4-OH-benzoate through the protocatechuate pathway (pobA and pcaHG) are also negatively modulated by Crc. However, the pathway for phenylacetate appeared to be unaffected by Crc. These results expand the influence of Crc to pathways used to assimilate several aromatic compounds, which highlights its importance as a master regulator of carbon metabolism in P. putida.
Authors:
Gracia Morales; Juan Francisco Linares; Ana Beloso; Juan Pablo Albar; José Luis Martínez; Fernando Rojo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  186     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-19     Completed Date:  2004-03-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1337-44     Citation Subset:  IM    
Affiliation:
Departamento de Biotecnología Microbiana. Servicio de Proteómica, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Chromosomes, Bacterial
Culture Media
Gene Deletion
Gene Expression Regulation, Bacterial*
Hydrocarbons, Aromatic / metabolism*
Proteome
Pseudomonas putida / genetics,  metabolism*
Repressor Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/Hydrocarbons, Aromatic; 0/Proteome; 0/Repressor Proteins; 0/crc protein, Pseudomonas
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