Document Detail


Pseudomonas fluorescens N21.4 metabolites enhance secondary metabolism isoflavones in soybean (Glycine max) calli cultures.
MedLine Citation:
PMID:  23039196     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Phytopharmaceuticals are plant secondary metabolites strongly inducible and especially sensitive to biotic changes. Plant cell cultures are a good alternative to obtain secondary metabolites, in case effective stimulation can be achieved. In this study metabolic elicitors from two rhizobacteria able to enhance isoflavone content in soybean seedlings, were tested on three different soybean calli cell lines. Results show that metabolic elicitors from Chryseobacterium balustinum Aur9 were not effective. However, there are at least two different metabolic elicitors from Pseudomonas fluorescens N21.4, one under 10 kDa and another over 10 kDa, that trigger isoflavone metabolism in the 3 cell lines with different isoflavone content. Elicitors from N21.4 achieved total isoflavones increases up to 29.7% (0.205 mg/g), 64.5% (0.487 mg/g) and 23.4% (0.726 mg/g) in the low, intermediate and high yield lines, respectively. Therefore, these elicitors have a great potential to enhance isoflavone production in cell cultures for development of functional ingredients.
Authors:
Elena Algar; Francisco Javier Gutierrez-Mañero; Alfonso Bonilla; Jose Antonio Lucas; Wojtek Radzki; Beatriz Ramos-Solano
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-6
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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