Document Detail


Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirements.
MedLine Citation:
PMID:  18390654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas aeruginosa demonstrates type IV pilus-mediated directional twitching motility up a gradient of phosphatidylethanolamine (PE). Only one of four extracellular phospholipases C of P. aeruginosa (i.e., PlcB), while not required for twitching motility per se, is required for twitching-mediated migration up a gradient of PE or phosphatidylcholine. Whether other lipid metabolism genes are associated with this behavior was assessed by analysis of transcription during twitching up a PE gradient in comparison to transcription during twitching in the absence of any externally applied phospholipid. Data support the hypothesis that PE is further degraded and that the long-chain fatty acid (LCFA) moieties of PE are completely metabolized via beta-oxidation and the glyoxylate shunt. It was discovered that P. aeruginosa exhibits twitching-mediated chemotaxis toward unsaturated LCFAs (e.g., oleic acid), but not saturated LCFAs (e.g., stearic acid) of corresponding lengths. Analysis of mutants that are deficient in glyoxylate shunt enzymes, specifically isocitrate lyase (DeltaaceA) and malate synthase (DeltaaceB), suggested that the complete metabolism of LCFAs through this pathway was required for the migration of P. aeruginosa up a gradient of PE or unsaturated LCFAs. At this point, our data suggested that this process should be classified as energy taxis. However, further evaluation of the ability of the DeltaaceA and DeltaaceB mutants to migrate up a gradient of PE or unsaturated LCFAs in the presence of an alternative energy source clearly indicated that metabolism of LCFAs for energy is not required for chemotaxis toward these compounds.
Authors:
Rhea M Miller; Andrew P Tomaras; Adam P Barker; Dennis R Voelker; Edward D Chan; Adriana I Vasil; Michael L Vasil
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-04-04
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-07-03     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4038-49     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Colorado-Denver, Aurora, CO 80045, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Bronchoalveolar Lavage Fluid / chemistry
Chemotaxis / physiology*
Fatty Acids / chemistry,  metabolism*
Fimbriae, Bacterial / physiology
Gene Expression Regulation, Bacterial / physiology
Humans
Molecular Structure
Oligonucleotide Array Sequence Analysis
Phospholipids / chemistry,  metabolism*
Pseudomonas aeruginosa / genetics,  physiology*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
AI15940/AI/NIAID NIH HHS; HL06208/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Fatty Acids; 0/Phospholipids
Comments/Corrections

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