| Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirements. | |
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MedLine Citation:
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PMID: 18390654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pseudomonas aeruginosa demonstrates type IV pilus-mediated directional twitching motility up a gradient of phosphatidylethanolamine (PE). Only one of four extracellular phospholipases C of P. aeruginosa (i.e., PlcB), while not required for twitching motility per se, is required for twitching-mediated migration up a gradient of PE or phosphatidylcholine. Whether other lipid metabolism genes are associated with this behavior was assessed by analysis of transcription during twitching up a PE gradient in comparison to transcription during twitching in the absence of any externally applied phospholipid. Data support the hypothesis that PE is further degraded and that the long-chain fatty acid (LCFA) moieties of PE are completely metabolized via beta-oxidation and the glyoxylate shunt. It was discovered that P. aeruginosa exhibits twitching-mediated chemotaxis toward unsaturated LCFAs (e.g., oleic acid), but not saturated LCFAs (e.g., stearic acid) of corresponding lengths. Analysis of mutants that are deficient in glyoxylate shunt enzymes, specifically isocitrate lyase (DeltaaceA) and malate synthase (DeltaaceB), suggested that the complete metabolism of LCFAs through this pathway was required for the migration of P. aeruginosa up a gradient of PE or unsaturated LCFAs. At this point, our data suggested that this process should be classified as energy taxis. However, further evaluation of the ability of the DeltaaceA and DeltaaceB mutants to migrate up a gradient of PE or unsaturated LCFAs in the presence of an alternative energy source clearly indicated that metabolism of LCFAs for energy is not required for chemotaxis toward these compounds. |
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Authors:
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Rhea M Miller; Andrew P Tomaras; Adam P Barker; Dennis R Voelker; Edward D Chan; Adriana I Vasil; Michael L Vasil |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-04-04 |
Journal Detail:
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Title: Journal of bacteriology Volume: 190 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-16 Completed Date: 2008-07-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 4038-49 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Colorado-Denver, Aurora, CO 80045, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism Bronchoalveolar Lavage Fluid / chemistry Chemotaxis / physiology* Fatty Acids / chemistry, metabolism* Fimbriae, Bacterial / physiology Gene Expression Regulation, Bacterial / physiology Humans Molecular Structure Oligonucleotide Array Sequence Analysis Phospholipids / chemistry, metabolism* Pseudomonas aeruginosa / genetics, physiology* Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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AI15940/AI/NIAID NIH HHS; HL06208/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Fatty Acids; 0/Phospholipids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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