Document Detail


The Pseudomonas aeruginosa autoinducer dodecanoyl-homoserine lactone inhibits the putrescine synthesis in human cells.
MedLine Citation:
PMID:  18452426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas aeruginosa uses acyl-homoserine lactones to coordinate gene transcription in a process called quorum sensing (QS). The QS molecules C4-HSL and C12-oxo-HSL are synthesized from the universal precursor S-adenosyl methionine, which is also a precursor of polyamines in human cells. Polyamines are required for mitotic cell division and peak during this phase. The polyamine putrescine is synthesized by ornithine decarboxylase (ODC) as a rate-limiting step. The ODC enzyme concentration also peaks during the mitotic phase. This peak is mediated by translation of ODC mRNA by the ITAF45 protein, which translocates from the nuclear compartment to the cytoplasm in a phosphorylation-dependent manner. We observed that C12-HSL-treated human epidermal cells had a higher cytoplasm-to-nuclear ITAF45 protein concentration and this translocation was dependent on the dephosphorylation of ITAF45. Finally, C12-HSL-treated cells also had a time-course-dependent higher concentration of ODC mRNA. Based on these mitotic markers, more human cells were apparently trapped in the mitotic phase when treated with C12-HSL. This should normally imply higher levels of putrescine. However, C12-HSL-treated human cells had a significantly lower concentration of putrescine and displayed a lower cell proliferation rate. In conclusion, the P. aeruginosa autoinducer C12-oxo-HSL apparently arrests human cells in the mitotic phase by lowering the concentration of putrescine.
Authors:
Søren Kristiansen; Thomas Bjarnsholt; Dinah Adeltoft; Peter Ifversen; Michael Givskov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  116     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-02     Completed Date:  2008-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  361-71     Citation Subset:  IM    
Affiliation:
Wound & Skin Care, Coloplast, Humlebaek, Denmark. dkskn@coloplast.com
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Division / physiology
Cell Line
Cell Proliferation
Growth Inhibitors / physiology
Homoserine / analogs & derivatives*,  physiology
Humans
Lactones
Molecular Sequence Data
Ornithine Decarboxylase / physiology
Pseudomonas aeruginosa / genetics,  pathogenicity,  physiology*
Putrescine / antagonists & inhibitors*,  biosynthesis*
Quorum Sensing / physiology
Chemical
Reg. No./Substance:
0/Growth Inhibitors; 0/Lactones; 110-60-1/Putrescine; 152833-54-0/Pseudomonas aeruginosa autoinducer; 498-19-1/Homoserine; EC 4.1.1.17/Ornithine Decarboxylase

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