Document Detail


Pseudomonas aeruginosa accentuates epithelial-to-mesenchymal transition in the airway.
MedLine Citation:
PMID:  20847079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epithelial-to-mesenchymal transition (EMT) has been implicated in the dysregulated epithelial wound repair that contributes to obliterative bronchiolitis (OB) after lung transplantation. Acquisition of Pseudomonas aeruginosa in the transplanted airway has been shown to be a risk factor for the development of OB. We investigated the potential of P. aeruginosa to drive EMT in primary bronchial epithelial cells (PBECs) isolated from lung transplant recipients. Changes in the expression of epithelial and mesenchymal markers was assessed in cells challenged with clinical isolates of P. aeruginosa or co-cultured with P. aeruginosa-activated monocytic cells (THP-1) in the presence or absence of transforming growth factor (TGF)-β1. P. aeruginosa did not drive or accentuate TGF-β1-driven EMT directly. Co-culturing P. aeruginosa-activated THP-1 cells with PBECs did not drive EMT. However, co-culturing P. aeruginosa-activated THP-1 cells with PBECs significantly accentuated TGF-β1-driven EMT. P. aeruginosa, via the activation of monocytic cells, can accentuate TGF-β1-driven EMT. These in vitro observations may help explain the in vivo clinical observation of a link between acquisition of P. aeruginosa and an increased risk of developing OB.
Authors:
L A Borthwick; S S Sunny; V Oliphant; J Perry; M Brodlie; G E Johnson; C Ward; K Gould; P A Corris; A De Soyza; A J Fisher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-16
Journal Detail:
Title:  The European respiratory journal     Volume:  37     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-31     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1237-47     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bronchi / drug effects,  microbiology
Bronchiolitis Obliterans / microbiology
Cell Line
Cells, Cultured
Coculture Techniques
Epithelial-Mesenchymal Transition*
Humans
Lung Transplantation
Macrophages, Alveolar / drug effects
Monocytes / drug effects
Pseudomonas Infections / complications,  metabolism
Pseudomonas aeruginosa*
Transforming Growth Factor beta1 / metabolism,  pharmacology
Grant Support
ID/Acronym/Agency:
G0500705//Medical Research Council; G0700861//Medical Research Council
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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