Document Detail

The Pseudomonas aeruginosa exopolysaccharide Psl facilitates surface adherence and NF-kappaB activation in A549 cells.
MedLine Citation:
PMID:  20802825     Owner:  NLM     Status:  MEDLINE    
In order for the opportunistic Gram-negative pathogen Pseudomonas aeruginosa to cause an airway infection, the pathogen interacts with epithelial cells and the overlying mucous layer. We examined the contribution of the biofilm polysaccharide Psl to epithelial cell adherence and the impact of Psl on proinflammatory signaling by flagellin. Psl has been implicated in the initial attachment of P. aeruginosa to biotic and abiotic surfaces, but its direct role in pathogenesis has not been evaluated (L. Ma, K. D. Jackson, R. M. Landry, M. R. Parsek, and D. J. Wozniak, J. Bacteriol. 188:8213-8221, 2006). Using an NF-kappaB luciferase reporter system in the human epithelial cell line A549, we show that both Psl and flagellin are necessary for full activation of NF-kappaB and production of the interleukin 8 (IL-8) chemokine. We demonstrate that Psl does not directly stimulate NF-kappaB activity, but indirectly as a result of increasing contact between bacterial cells and epithelial cells, it facilitates flagellin-mediated proinflammatory signaling. We confirm differential adherence of Psl and/or flagellin mutants by scanning electron microscopy and identify Psl-dependent membrane structures that may participate in adherence. Although we hypothesized that Psl would protect P. aeruginosa from recognition by the epithelial cell line A549, we instead observed a positive role for Psl in flagellin-mediated NF-kappaB activation, likely as a result of increasing contact between bacterial cells and epithelial cells.
Matthew S Byrd; Bing Pang; Meenu Mishra; W Edward Swords; Daniel J Wozniak
Related Documents :
6365705 - Adherence of group b streptococci isolated from man and cattle to human and bovine epit...
17385545 - Studies on the antibacterial activities and mechanisms of chitosan obtained from cuticl...
8757825 - Invasion of epithelial cells by actinobacillus actinomycetemcomitans: a dynamic, multis...
16491355 - Effects of nitrate treatment on a mixed species, oil field microbial biofilm.
2852075 - Cell and molecular studies of renin secretion.
7492335 - Effects of hydration state on the synthesis and secretion of triacylglycerol by isolate...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-29
Journal Detail:
Title:  mBio     Volume:  1     ISSN:  2150-7511     ISO Abbreviation:  MBio     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-30     Completed Date:  2011-08-12     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101519231     Medline TA:  MBio     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Adhesion*
Cell Line
Epithelial Cells / immunology,  microbiology
Flagellin / genetics,  immunology
NF-kappa B / immunology*
Polysaccharides, Bacterial / immunology*
Pseudomonas Infections / immunology*,  microbiology
Pseudomonas aeruginosa / genetics,  immunology,  physiology*
Grant Support
Reg. No./Substance:
0/NF-kappa B; 0/Polysaccharides, Bacterial; 12777-81-0/Flagellin; 128531-82-8/exopolysaccharide, Pseudomonas

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Robust stimulation of humoral and cellular immune responses following vaccination with antigen-loade...
Next Document:  The combination of rifampin plus moxifloxacin is synergistic for suppression of resistance but antag...