| The Pseudomonas aeruginosa exopolysaccharide Psl facilitates surface adherence and NF-kappaB activation in A549 cells. | |
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MedLine Citation:
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PMID: 20802825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order for the opportunistic Gram-negative pathogen Pseudomonas aeruginosa to cause an airway infection, the pathogen interacts with epithelial cells and the overlying mucous layer. We examined the contribution of the biofilm polysaccharide Psl to epithelial cell adherence and the impact of Psl on proinflammatory signaling by flagellin. Psl has been implicated in the initial attachment of P. aeruginosa to biotic and abiotic surfaces, but its direct role in pathogenesis has not been evaluated (L. Ma, K. D. Jackson, R. M. Landry, M. R. Parsek, and D. J. Wozniak, J. Bacteriol. 188:8213-8221, 2006). Using an NF-kappaB luciferase reporter system in the human epithelial cell line A549, we show that both Psl and flagellin are necessary for full activation of NF-kappaB and production of the interleukin 8 (IL-8) chemokine. We demonstrate that Psl does not directly stimulate NF-kappaB activity, but indirectly as a result of increasing contact between bacterial cells and epithelial cells, it facilitates flagellin-mediated proinflammatory signaling. We confirm differential adherence of Psl and/or flagellin mutants by scanning electron microscopy and identify Psl-dependent membrane structures that may participate in adherence. Although we hypothesized that Psl would protect P. aeruginosa from recognition by the epithelial cell line A549, we instead observed a positive role for Psl in flagellin-mediated NF-kappaB activation, likely as a result of increasing contact between bacterial cells and epithelial cells. |
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Authors:
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Matthew S Byrd; Bing Pang; Meenu Mishra; W Edward Swords; Daniel J Wozniak |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-06-29 |
Journal Detail:
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Title: mBio Volume: 1 ISSN: 2150-7511 ISO Abbreviation: MBio Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-30 Completed Date: 2011-08-12 Revised Date: 2011-10-12 |
Medline Journal Info:
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Nlm Unique ID: 101519231 Medline TA: MBio Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Wake Forest University Health Sciences, Winston-Salem, North Carolina, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion* Cell Line Epithelial Cells / immunology, microbiology Flagellin / genetics, immunology Humans NF-kappa B / immunology* Polysaccharides, Bacterial / immunology* Pseudomonas Infections / immunology*, microbiology Pseudomonas aeruginosa / genetics, immunology, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI061396/AI/NIAID NIH HHS; AI07870002/AI/NIAID NIH HHS; HL058334/HL/NHLBI NIH HHS; R01 HL058334-13/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/NF-kappa B; 0/Polysaccharides, Bacterial; 12777-81-0/Flagellin; 128531-82-8/exopolysaccharide, Pseudomonas |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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