Document Detail


The Pseudomonas aeruginosa exopolysaccharide Psl facilitates surface adherence and NF-kappaB activation in A549 cells.
MedLine Citation:
PMID:  20802825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order for the opportunistic Gram-negative pathogen Pseudomonas aeruginosa to cause an airway infection, the pathogen interacts with epithelial cells and the overlying mucous layer. We examined the contribution of the biofilm polysaccharide Psl to epithelial cell adherence and the impact of Psl on proinflammatory signaling by flagellin. Psl has been implicated in the initial attachment of P. aeruginosa to biotic and abiotic surfaces, but its direct role in pathogenesis has not been evaluated (L. Ma, K. D. Jackson, R. M. Landry, M. R. Parsek, and D. J. Wozniak, J. Bacteriol. 188:8213-8221, 2006). Using an NF-kappaB luciferase reporter system in the human epithelial cell line A549, we show that both Psl and flagellin are necessary for full activation of NF-kappaB and production of the interleukin 8 (IL-8) chemokine. We demonstrate that Psl does not directly stimulate NF-kappaB activity, but indirectly as a result of increasing contact between bacterial cells and epithelial cells, it facilitates flagellin-mediated proinflammatory signaling. We confirm differential adherence of Psl and/or flagellin mutants by scanning electron microscopy and identify Psl-dependent membrane structures that may participate in adherence. Although we hypothesized that Psl would protect P. aeruginosa from recognition by the epithelial cell line A549, we instead observed a positive role for Psl in flagellin-mediated NF-kappaB activation, likely as a result of increasing contact between bacterial cells and epithelial cells.
Authors:
Matthew S Byrd; Bing Pang; Meenu Mishra; W Edward Swords; Daniel J Wozniak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-29
Journal Detail:
Title:  mBio     Volume:  1     ISSN:  2150-7511     ISO Abbreviation:  MBio     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-30     Completed Date:  2011-08-12     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101519231     Medline TA:  MBio     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion*
Cell Line
Epithelial Cells / immunology,  microbiology
Flagellin / genetics,  immunology
Humans
NF-kappa B / immunology*
Polysaccharides, Bacterial / immunology*
Pseudomonas Infections / immunology*,  microbiology
Pseudomonas aeruginosa / genetics,  immunology,  physiology*
Grant Support
ID/Acronym/Agency:
AI061396/AI/NIAID NIH HHS; AI07870002/AI/NIAID NIH HHS; HL058334/HL/NHLBI NIH HHS; R01 HL058334/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/NF-kappa B; 0/Polysaccharides, Bacterial; 12777-81-0/Flagellin; 128531-82-8/exopolysaccharide, Pseudomonas
Comments/Corrections

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