Document Detail


Pseudolaric acid B induces apoptosis via activation of c-Jun N-terminal kinase and caspase-3 in HeLa cells.
MedLine Citation:
PMID:  15665588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudolaric acid B was isolated from Pseudolarix kaempferi Gordon (Pinaceae) and was evaluated for the anti-cancer effect in HeLa cells. We ob-served that pseudolaric acid B inhibited cell proliferation and induced apoptosis in a time- and dose-dependent manner. HeLa cells treated with pseudolaric acid B showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. JNK inhibitor, SP600125,markedly inhibited pseudolaric acid B-induced celldeath. In addition, Bcl-2 expression was down-regulated while Bax protein level was up-regulated.Caspase-3 inhibitor, z-DEVD-fmk, partially blocked pseudolaric acid B-induced cell death, and the expression of two classical caspase substrates,PARP and ICAD, were both decreased in a time-dependent manner, indicative of downstream cas-pase activation.
Authors:
Xianfeng Gong; Minwei Wang; Zhen Wu; Shin-ichi Tashiro; Satoshi Onodera; Takashi Ikejima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  36     ISSN:  1226-3613     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-24     Completed Date:  2005-06-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  551-6     Citation Subset:  IM    
Affiliation:
China-Japan Research Institute of Medical Pharmaceutical Sciences, Department of Pharmacology, Shenyang Pharmaceutical University, China.
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MeSH Terms
Descriptor/Qualifier:
Anthracenes / pharmacology
Apoptosis*
Caspase 3
Caspases / antagonists & inhibitors,  metabolism*
Cell Proliferation / drug effects
Cysteine Proteinase Inhibitors / pharmacology
Diterpenes / pharmacology*
Down-Regulation
Enzyme Activation
Hela Cells
Humans
JNK Mitogen-Activated Protein Kinases / drug effects,  metabolism*
Oligopeptides / pharmacology
Protein Kinase Inhibitors / pharmacology
Proto-Oncogene Proteins c-bcl-2 / metabolism
Signal Transduction / drug effects*
Up-Regulation
bcl-2-Associated X Protein
bcl-X Protein
Chemical
Reg. No./Substance:
0/Anthracenes; 0/BAX protein, human; 0/BCL2L1 protein, human; 0/Cysteine Proteinase Inhibitors; 0/Diterpenes; 0/Oligopeptides; 0/Protein Kinase Inhibitors; 0/Proto-Oncogene Proteins c-bcl-2; 0/anthra(1,9-cd)pyrazol-6(2H)-one; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; 0/benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; 82508-31-4/pseudolaric acid B; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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