Document Detail

'Pseudohypouricosuria' in alcaptonuria: homogentisic acid interference in the measurement of urinary uric acid with the uricase-peroxidase reaction.
MedLine Citation:
PMID:  10456213     Owner:  NLM     Status:  MEDLINE    
Urinary excretion of uric acid was found to be extremely low in a 58-year-old female patient with alcaptonuria. This was due to interference with the uricase-peroxidase method used, because analysis using high-performance liquid chromatography (HPLC) showed a normal urinary concentration of uric acid. In vitro experiments demonstrated that a high concentration of homogentisic acid in the patient's urine inhibited the peroxidase reaction, possibly due to inhibition of the colour development of 3-methyl-N-ethyl-N-(beta-hydroxyethyl)aniline (MEHA) and 4-aminoantipyrine, via the peroxidase reaction. A homogentisic acid concentration equivalent to that in plasma did not affect the uricase-peroxidase reaction. This result suggests that any assay based on a peroxidase method is affected by a high urinary concentration of homogentisic acid in patients with alcaptonuria.
Y Moriwaki; T Yamamoto; Y Nasako; H Ohata; S Takahashi; Z Tsutsumi; J Yamakita; K Higashino
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Annals of clinical biochemistry     Volume:  36 ( Pt 4)     ISSN:  0004-5632     ISO Abbreviation:  Ann. Clin. Biochem.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-22     Completed Date:  1999-09-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0324055     Medline TA:  Ann Clin Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  501-3     Citation Subset:  IM    
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
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MeSH Terms
Alkaptonuria / complications,  urine*
Homogentisic Acid / urine*
Middle Aged
Peroxidase / metabolism
Urate Oxidase / metabolism
Uric Acid / urine*
Reg. No./Substance:
451-13-8/Homogentisic Acid; 69-93-2/Uric Acid; EC; EC Oxidase

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