Document Detail


Pseudo-template molecularly imprinted polymer for selective screening of trace beta-lactam antibiotics in river and tap water.
MedLine Citation:
PMID:  20630531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the potential risks associated with the environmental exposure of beta-lactam antibiotics (BLAs), the monitoring of the occurrence, distribution, and fate of these emerging contaminants in the environment is required. Herein, we demonstrate a molecularly imprinted solid-phase extraction (MISPE) method for selective and reliable screening of trace BLAs in river and tap water. By developing a low-temperature photopolymerization, highly selective molecularly imprinted polymers (MIPs) for five BLAs (penicillin G, amoxicillin, ampicillin, nafcillin and mezlocillin) were synthesized. Nafcillin was chosen as a pseudo template to make the MIP sorbent (Nafc-MIP), which was used in pseudo-template MISPE for preconcentration of the other four BLAs from river and tap water. The application of pseudo-template MISPE overcomes the template bleeding, which significantly elevates the sample background and restricts the application of MIP for detection of the target BLA below 2 microg/L. The average recoveries of BLAs are in the range of 60-90% when Nafc-MIP was adopted as the selective MISPE sorbent. The developed method was validated, and applied to the screening of trace beta-lactam antibiotics in river and tap water. The linearity of the calibration curve for each BLA was observed over the range of 0.1-20 microg/L (r>0.998). The beta-lactam antibiotics were found within the range of 0-9.56 microg/L in river water at the downstream of antibiotics manufacturers, and none were detected in the tap water.
Authors:
Junfa Yin; Zihui Meng; Mingjun Du; Cong Liu; Maoyong Song; Hailin Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-25
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5420-6     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Affiliation:
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Linear Models
Molecular Imprinting*
Penicillins / chemistry,  isolation & purification*
Photochemistry / methods
Reproducibility of Results
Rivers / chemistry*
Sensitivity and Specificity
Water Supply / analysis*
Chemical
Reg. No./Substance:
0/Penicillins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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